三阴性乳腺癌中DJ-1和HSP27的表达及其临床意义

摘要

Objective:To investigate the expression and clinical significance of DJ-1 and HSP27 in triple-negative breast cancer (TNBC).Methods:Total of 185 cases of TNBC paraffin-embedded tissue samples and 120 cases of normal mammary gland tissue samples were collected in Xijing Hospital.The expression of DJ-1 and HSP27 protein in TNBC tissue and normal breast tissue was detected by immunohistochemical staining.And analyze their relationship with the TNBC clinicopathological features.Results:The positive expression rates of DJ-1 and HSP27 in TNBC were 74.1％ and 67.6％ respectively.In contrast,the positive expression rates of DJ-1 and HSP27 in normal breast tissues were 5.0％ and 20.8％.Compared with normal tissues,the positive expression rates of DJ-1 and HSP27 were significantly increased (P ＜ 0.05).The expression of DJ-1 in TNBC was significantly associated with lymph node metastasis,differentiation and TNM stages (P ＜ 0.05).The expression of HSP27 in TNBC was significantly associated with tumor differentiation (P ＜ 0.05).In 185 cases of TNBC,DJ-1 expression was positive correlated with HSP27 expression(r =0.519,P ＜ 0.05).Conclusion:DJ-1 and HSP27 were over-expressed in TNBC.DJ-1 might be related to the malignant proliferation,lymph node metastasis and invasion of TNBC.HSP27 might be related to the differentiation level of malignant tumor cells of TNBC.DJ-1 had cooperative effect with HSP27 in TNBC.%目的:探讨三阴性乳腺癌(TNBC)中DJ-1和HSP27的表达及其临床意义.方法:收集第四军医大学西京医院TNBC石蜡组织标本185例和正常乳腺石蜡组织标本120例,采用免疫组织化学SP法检测DJ-1和HSP27蛋白在TNBC组织和正常乳腺组织中的表达,并分析两者与TNBC临床病理学特征的关系.结果:185例TNBC组织中有137例呈DJ-1阳性表达,表达率为74.1％；有125例呈HSP27阳性表达,表达率为67.6％.120例正常乳腺组织中,6例DJ-1呈弱阳性表达,表达率为5.0％,而25例呈HSP27阳性表达,表达率为20.8％.TNBC组织中DJ-1和HSP27阳性表达率明显高于正常乳腺组织(P＜0.05).DJ-1在TNBC组织中的表达与腋窝淋巴结转移、组织学分化、TNM分期、脉管癌栓密切相关(P＜0.05),HSP27在TNBC组织中的表达与组织学分化密切相关(P＜0.05).185例TNBC组织中,DJ-1和HSP27蛋白的表达之间呈正相关(r=0.519,P＜0.05).结论:TNBC组织中存在DJ-1和HSP27的高表达,DJ-1对TNBC患者的恶性增殖、淋巴结转移、侵袭性强的生物学行为有重要影响,HSP27对TNBC患者的肿瘤细胞恶性分化程度有重要影响,且两者间存在协同作用.