miR-152影响NK细胞对JEG-3细胞的杀伤效应

摘要

目的:研究miR-152分子对NK细胞杀伤JEG-3细胞效应的影响.方法:在滋养细胞肿瘤细胞系JEG-3细胞中分别转染pre-miR-152(实验组),Cy3标记的pre-miRNA-control(阴性对照),空白对照(NC)为未转染的JEG-3细胞.转染48h进行NK细胞的细胞毒测定,观察各组NK细胞对JEG-3细胞的杀伤效应.结果:与对照组相比,实验组NK细胞对JEG-3细胞的平均杀伤率显著增高(P＜0.05),且随着效靶比的增高,杀伤率也增大.空白对照组与阴性对照组之间的NK细胞杀伤能力无显著差异(P＞0.05).结论:miR-152可以提高NK细胞对滋养细胞的杀伤作用.%Objective:To investigate the effect of miR-152 on NK cell mediated cytolysis in JEG-3 cells.Methods:The JEG-3 cells were transfected with pre-miR-152 (test group),Cy3 dye labeled pre-miRNA-control (negative control),respectively.JEG-3 cells transfected with nothing were blank control (NC).48 hours after transfction,cells were used for NK cell cytotoxicity assay to investigate the NK cell mediated cytolysis in JEG-3 cells.Results:After pre-miR-152 transfection,the lyticactivity of NK cells toward JEG-3 cells was dramatically enhanced compared with that in the control groups at each E/T ratio (NK cells to JEG-3 cell ratio,P ＜ 0.05),and the cytotoxicity increased proportionately to an increase in the E/T ratio.There was no difference between negative control group and blank control group (P ＞ 0.05).Conclusion:This study reveals that overexpression of miR-152 could lead to increased NK cell mediated cytolysis in JEG-3 cells,suggesting that miR-152 may function as an immune system enhancer through up-regulating NK cell mediated cytolysis of host cells.