GRP94在HBV感染的人肝细胞肝癌中的表达及意义

摘要

目的：探讨葡萄糖调节蛋白（ glucose-regulated protein 94，GRP94）在乙型肝炎病毒（ hepatitis B virus， HBV）感染的人肝细胞肝癌（ human hepatocellular carcinomas，HCC）中的表达及其意义。方法：酶联免疫吸附试验（ enzyme-linked immunosorbent assay，ELISA）和实时荧光定量 PCR（ real -time fluorescence quantitative polymerase chain reaction，RTQF-PCR）方法来检测HCC患者血清。采用免疫组织化学（ immunohistochemistry， IHC）方法、蛋白质印迹（ Western blot）方法及半定量逆转录聚合酶链反应（ reverse transcrition -polymerase chain reaction，RT-PCR），来探讨GRP94在HBV病毒感染的HCC和癌旁组织中的表达。结果：GRP94在癌旁组织中低表达，而在HCC中高表达，两者在Western blot、IHC及RT-PCR中的表达，其差异有统计学意义。同时HCC中GRP94的表达强度与肝癌患者肿瘤TNM分期（P=0.02）、肿瘤大小（P=0.01）、肿瘤结节的数目（P=0.02）、HBV-DNA的拷贝数值（P<0.01）和血清甲胎蛋白（alpha-fetal protein，AFP）水平（P=0.02）相关，但与患者的性别、年龄差异无关。结论：GRP94与HCC临床病理特征存在一定的相关性，对HCC治疗、预后判定具有一定指导意义。%Objective:To investigate the expression and significance of glucose-regulated protein 94 in human hepatocellular carcinomas( HCC)with hepatitis B virus( HBV)infections. Methods:Enzyme-linked immunosorbent assay( ELISA)and real-time fluorescence quantitative polymerase chain reaction( RTFQ-PCR)were performed on HCC serums. Immunohistochemistry assay( IHC)and Western Blot and reverse transcription-polymerase chain reac-tion( RT-PCR)were performed on HCC tissues. Results:High GRP94 expression was presented with HCC tissues and low GRP94 was expressed in adjacent cancer tissues by IHC analysis performing on formalin-fixed paraffin sec-tions of 50 specimens. Statistical analysis showed that GRP94 expression correlated significantly with TNM stage( P=0. 02),tumor size(P=0. 01),the number of tumor nodules(P=0. 02),HBV-DNA copy values(P<0. 01),and serum AFP level(P=0. 02),whereas GRP94 expressions were uncorrelated significantly with patientˊs gender and age. Conclusion:GRP94 expression has a certain correlation with HCC. These findings will provide another further guidance significance of prognosis and treatment for HCC.