mTOR通路对神经母细胞瘤SH-SY5 Y细胞自噬作用的影响

摘要

目的：研究雷帕霉素靶蛋白（ mammalian target of rapamycin，mTOR）通路对神经母细胞瘤SH-SY5Y细胞自体吞噬作用的影响。方法：应用不同浓度的雷帕霉素作用于神经母细胞瘤细胞，采用实时荧光定量PCR检测雷帕霉素作用前后神经母细胞瘤细胞株的mTOR、LC3及Beclin 1 mRNA表达情况。结果：雷帕霉素能抑制mTOR表达且呈现量效依赖关系，不同浓度的雷帕霉素分别对SH-SY5Y细胞作用72h后，mTOR mR-NA的表达随浓度增加逐渐下降。而LC3及Beclin 1 mRNA的表达随浓度增加逐渐上升，神经母细胞瘤中mTOR与LC3及Beclin 1的表达呈负相关。结论：雷帕霉素通过抑制mTOR信号通路促进神经母细胞瘤的自体吞噬作用，动态观察mTOR可作为神经母细胞瘤治疗效果监测的指标之一。%Objective:To investigate the effect of mTOR signaling pathway on autophagy of neuroblustoma SH-SY5Y cells. Methods:Different concertration rapamycin were administrated to neuroblustoma SH-SY5Y cells. Real-time quantitative PCR was used to detect the expressions of mTOR,LC3 and Beclin 1 mRNA in neuroblustoma SH-SY5Y cells. Results:Rapamycin inhibited the expression of mTOR. Defferent concentrations of rapamycin affected on SH-SY5Y cell line,the mRNA expression of mTOR decreased in dose-dependent manner,but the mRNA ex-pression of LC3 and Beclin 1 in SH-SY5Y cell line increased in dose-dependent manner. The expression of mTOR was negatively correlated with the expressions of LC3 and Beclin 1 in SH-SY5Y cells. Conclusion:Inhibition of the mTOR signaling pathway can promote autophagy of neuroblustoma SH - SY5Y cells. The dynamic examination of mTOR may be used as an index of the efficacy of treatment of neuroblastoma.