艾灸抑制小鼠皮下移植瘤生长的活体成像观察

摘要

目的：观察艾灸对小鼠皮下移植瘤的抑制作用。方法：通过慢病毒转染，构建能稳定表达荧光素酶基因的乳腺癌细胞株，建立小鼠皮下移植瘤模型后通过随机区组方法设为对照组和治疗组，治疗组又分为关元组、大椎组。治疗组艾灸隔天一次，每次10min，共7次，对照组不做处理。通过活体成像系统以及体外测量，监测肿瘤生长情况。治疗终点，处死小鼠，HE染色观察肿瘤的病理形态学变化。结果：建立能稳定表达荧光素酶基因的乳腺癌细胞株及小鼠皮下移植瘤模型，细胞数与荧光强度呈线性关系（ r=0.899）。活体成像显示：治疗组小鼠荧光强度较对照组明显减弱。体外测量，治疗组肿瘤体积及重量明显小于对照组（P%Objective:To investigate the inhibitory effect of moxibustion in mice bearing subcutaneous cancer cell lines. Methods:Breast cancer cell line 4T1 with stable expression of luciferase gene(4T1/luc)were established by transfection via lentiviral vector. 4T1/luc cells were inoculated into the right leg of mice to prepare subcutaneous tumor model. A total of 15 tumor-bearing mouse were randomly divided into the following groups:the model control group and treatment group,the later includes guanyuan group and dazhui group. Mouse in dazhui and guanyuan group were treated with moxibustion,once another day for 10min,for a total of 7 times. The control group did nothing. The dynamic growth of subcutaneous tumor was observed using bioluminescence imaging system in vivo. At the end of the treatment,morphology of transplanted tumor tissue was observed by HE staining. Results:The stable 4T1/luc cells and subcutaneous tumor model were obtained. Bioluminescence imaging showed that fluorescent intensity of treated group was apparently lower than that of the control group. Compare with control group,the tomor volume and weight of treated group were significantly decreased(P <0. 05). The difference about the tomor volume and weight between guanyuan group and dazhui group had no statistic significance(P>0. 05). Compare with control group,the treated group had more inflammatory cells and eosinophilic increasing. Conclusion:Moxibustion shows potent anti-tumor ac-tivity in subcutaneous breast cancer. Bioluminescence imaging system may be helpful to provide a theoretical base for clinic use of moxibustion.