VEGF-C在宫颈癌细胞中的表达及与细胞增殖的关系

摘要

目的：研究VEGF-C在宫颈癌细胞中的表达及其对宫颈癌细胞增殖的影响。方法：采用反义技术，脂质体介导VEGF-C反义寡核苷酸转染人宫颈癌Hela细胞。应用荧光定量RT-PCR法、间接免疫荧光标记法，检测转染后细胞内VEGF-C在mRNA、蛋白水平的变化。MTT比色法检测细胞生长的变化。结果：脂质体介导VEGF-C反义寡核苷酸，转染宫颈癌Hela细胞的转染效率大于90%。转染了反义寡核苷酸的反义组VEGF-C在mRNA和蛋白水平上，较未转染组和正义组均明显下降（ P<0.05）。MTT法检测转染了VEGF-C反义寡核苷酸的细胞，明显看到反义组细胞的生长受到明显抑制。结论：VEGF-C可影响人宫颈癌Hela细胞增殖，本实验通过抑制VEGF-C，进而可阻断宫颈癌的淋巴转移，为实现靶向性基因治疗的可行性，提供了实验依据。%Objective:To study VEGF-C expression in cervical cancer cell and its relation with cervical cancer cell proliferation. Methods:VEGF-C antisense oligonucleotide was transfected into cervical carcinoma cell line Hela by LipofectamineTM2000 transfection reagent. The expression of protein and mRNA of VEGF-C were determined with FQ-RT-PCR,immunofluorescence using fluorescein isothiocyanate label and MTT assays. Results:After transfected by VEGF-C antisense oligonucleotide in vitro,the level of protein expression of VEGF-C and VEGF-C mRNA in Hela cell,were significantly decreased. The proliferation of Hela cell transfected by VEGF-C antisense oligonucleoti-de,was significantly decended. Conclusion:The function of VEGF-C gene of cervical cancer cell line Hela in tumor metastasis of lymoph node is not only stimulating lynphangiogenesis but also affecting the proliferation of tumor cell. The experimental study of VEGF-C in lymph node metastasis is the first step of inhibit cervical carcinoma lymph node metastasis.