CIK、DC-CIK细胞对神经母细胞瘤细胞杀伤作用的研究

摘要

Objective:To study the killing effects on neuroblastoma( NB)cells of cytokine-induced killer cells ( CIK)co-cultured with dendritic cells( DC). Methods:Peripheral blood mononuelear cells( PBMC)were isolated from healthy subjects and tumor patients. DC and CIK cells were induced by different cytokines. The phenotypes of CIK and DC cells before and after culture were determined by flow cytometry. The killing activity of different CIK groups against NB was determined by MTT. Results:The percentage of CD3+CD56+ cells as well as the cytotoxicity activity of CIK cells originated from PBMC of peripheral blood of healthy people were significantly higher than those of tumor patients(P<0. 05). In addition,compared with CIK cells,the DC-CIK cells presented a significantly higher killing effects on NB cells(P<0. 05). Conclusion:DC-CIK cell is a kind of immune cell which has a higher cyto-toxicity than that in CIK cell. CIK cells generated from PBMC of healthy subjects and tumor patients have significant differences,and provide further experimental basis for improving the clinical curative effect of CIK cells.%目的：研究细胞因子诱导的杀伤细胞（ CIK）与树突状细胞（ DC）共培养后对神经母细胞瘤（ neuro-blastoma，NB）细胞株的杀伤作用。方法：取健康人和肿瘤患者外周血单个核细胞（ PBMC），加入不同的细胞因子分别诱导出DC和CIK细胞，用流式细胞术测定诱导培养前后DC和CIK细胞的表型，MTT法测定不同组CIK细胞对NB细胞株的杀伤活性。结果：流式细胞仪检测健康人PBMC培养后CD3+CD56+淋巴细胞百分比以及对NB细胞株的杀伤活性均显著高于肿瘤患者（ P<0.05）。此外，与单纯CIK细胞相比，DC-CIK细胞具有更强的杀伤NB细胞株的活性（ P<0.05）。结论：DC-CIK细胞是一种细胞毒作用高于单纯CIK细胞的免疫活性细胞。健康人和肿瘤患者的PBMC经诱导培养获得的CIK细胞有显著差别，为临床进一步提高CIK细胞的治疗效果提供了实验依据。