结肠癌组织中Foxp3和TGF-β1的相互关系

摘要

目的：对结肠癌组织中Foxp3、TGF-β1蛋白进行检测，结合临床病理资料，分析其表达与患者临床病理资料及预后的关系。方法：收集56例结肠癌、癌旁组织标本和15例健康对照组的标本。应用免疫组化法检测56例结肠癌原发灶及癌旁组织和15例健康对照组织中Foxp3、TGF-β1蛋白的表达。结果：结肠癌患者组织Foxp3、TGF-β1阳性表达率均显著高于健康对照组和癌旁对照组（ P<0.01）。癌旁对照组Foxp3、TGF-β1阳性表达率高于健康对照组，有统计学意义（ P<0.05）。阳性表达率与淋巴结转移、临床Duke分期有关（P<0.05），与组织学分级、肿瘤大小无关（P>0.05）。Spearman等级相关分析表明，结肠癌组织Foxp3与TGF-β1蛋白表达呈正相关。结论：Foxp3、TGF-β1在结肠癌组织中表达增高，提示在结肠癌患者中，全身免疫状态与肿瘤的生长和侵袭密切相关，Foxp3、TGF-β1可作为判断患者预后的指标，并为结肠癌的靶向治疗提供新的途径。%Objective:To study the expression of Foxp3 and TGF-β1 protein in colon cancer and the relationship between their expression and clinical pathology and prognosis. Methods:To collect 56 cases of colon cancer and tissue adjacent to carcinoma specimens and the specimens of 15 cases of healthy controls. Using SP immunohistochemistry method to detect the expression of Foxp3 and TGF-β1 protein in 56 cases of primary colon cancer and paracancerous tissues and 15 cases of healthy control tissues. Results:The number of the Foxp3 and TGF-β1 positive cell in tissues of patients with colon cancer was significantly more than those in healthy controls and paracancerous tissue( P<0.05).Lymph node metastasis,Duke stage was positively correlated(P <0.05),but no relation with histological grade,tumor size(P>0. 05). Foxp3 colon carcinoma and the expression of TGF-β1 protein was significantly correla-ted. Conclusion:The expression of Foxp3 was highly correlated with the expression of TGF-β1 ,suggesting that sys-temic immune state was closely related to the growth and invasion of the tumor,Foxp3 and TGF-β1 protein can be used as a prognostic indicator,and it is possible that it can provide a new way for targeted therapy of colon cancer.