microRNA-99a 在非小细胞肺癌中的表达及临床意义

摘要

Objective:To investigate the expression of serum microRNA -99a in patients with NSCLC and in healthy people and to analyze the association of the expression with the cIinicopathologicaI features and prognosis. Methods:The expression levels of serum microRNA-99a in 70 patients with NSCLC and 60 healthy people were de-tected by using stem-loop real-time reverse transcription-polymerase chain reaction RT-PCR. Results:Expres-sion levels of microRNA-99a were downregulated in patients with NSCLC as compared with the healthy people(P<0. 05). The expression of microRNA-99a was associated with lymphatic metastasis,differentiation and TNM stages (P<0. 05). Low miR-99a expression was significantly correlated with overall survival(P<0. 05)of NSCLC. Con-clusion:The downregulation of serum microRNA-99a expression has relation with disease progression and microRNA-99a can serve as a potential therapeutic target for NSCLC.%目的：探讨microRNA-99a在非小细胞肺癌（ NSCLC）患者与正常人血清中的表达情况并分析其表达与临床病理特征及预后的关系。方法：收集70例非小细胞肺癌患者和60例正常人血清总RNA采用RT-PCR方法检测microRNA-99a的相对表达量，并分析其与临床病理资料和预后的相关性。结果：与正常人比较，血清microRNA-99a在非小细胞肺癌患者血清中表达下调，其表达与非小细胞肺癌患者的淋巴结转移、分化程度和TNM分期有显著相关性（ P<0.05），非小细胞肺癌血清中microRNA-99a低表达者生存时间明显低于高表达者（P<0.05）。结论：非小细胞肺癌患者血清中microRNA-99a的表达下调与病情进展具有一定的相关性，可能作为肺癌治疗的新靶点。