缺氧环境中Galectin-l对口腔鳞癌细胞增殖、侵袭与凋亡的影响

摘要

目的：探讨缺氧条件下Galectin-1对口腔鳞癌细胞Tca8113的生物学行为的影响。方法：siRNA干扰Galectin-1在Tca8113细胞中的表达；采用MTT法检测细胞的增殖；Real time-PCR分析Galectin-1 mR-NA和Bcl-2 mRNA的表达；Transwell小室观察各组细胞的侵袭能力；免疫荧光分析缺氧条件下细胞的凋亡情况。结果：siRNA-Galectin-l干扰组和200μmol/L氯化钴处理组的细胞出现了增殖抑制；用200μmol/L氯化钴处理Tca8113细胞后，Galectin-l mRNA的表达量上调（0．91±0．11，P＜0．05）；siRNA＋Tca8113细胞组Bcl-2 mRNA的表达量明显减低（0．16±0．02，P＜0．05）；siRNA-Galectin-l干扰组迁移出的细胞数（31±6）明显低于200μmol/L氯化钴＋Tca8113细胞组（43±6）和Tca8113细胞组（51±8，P＜0．05）；免疫荧光结果所示，siRNA＋200μmol/L氯化钴＋Tca8113细胞组的荧光强度最弱。结论：Galectin-1是反应肿瘤细胞缺氧的一个较为敏感的指标，可利用Galectin-1作为内在的缺氧标志物。%Objective:The biological effects of Galectin-l for oral squamous cell carcinoma cells in hypoxic envi-ronment were investigated.Methods:To interfere the expression of Galectin-1 in cells with siRNA,the proliferation of cells was detected by MTT methods;the expression of Galectin-1 mRNA and Bcl-2 mRNA were analysed by real time-PCR,using Transwell to detect the invasion ability;to analyse the condition of cell apoptosis in hypoxic by im-munofluorescence.Results:siRNA-Galectin-l group and 200μmol/L CoCl2 group appeared the inhibition of prolif-eration,the expression of Galectin-1 mRNA was significantly increased in 200μmol/L CoCl2 group (0.91 ±0.11,P<0.05),the expression of Bcl-2 mRNA was significantly reduced in siRNA group (0.16 ±0.02,P<0.05);the cell counts of siRNA-Galectin-l interference group (31 ±6)was significantly lower than 200μmol/L CoCl2 group (43 ±6)was Tca8113 cell group (51 ±8,P<0.05),the fluorescence intensity of siRNA and 200μmol/L CoCl2 and Tca8113 cell group were the weakest.Conclusion:Galectin-1 was a sensitive indicator with tumor cells in hypoxic environment,Galectin-1 maybe used as an inner oxygen marker.