趋化因子 CXCL16在胃癌中的表达及其临床病理学意义

摘要

目的：研究趋化因子 CXCL16在胃癌和正常黏膜中的表达情况及其关系，从而探讨 CXCL16在胃癌发生、发展中的作用及临床意义。方法：收集术中新鲜胃癌组织及对应的正常黏膜，进行免疫组化和 RT -PCR 实验检测 CXCL16蛋白和 mRNA 水平。其表达情况与各项临床病理学指标进行对比分析，同时进行生存分析。结果：CXCL16在28例新鲜标本中，胃癌组织中 CXCL16的水平明显高于癌旁正常组织。免疫组化显示 CXCL16主要表达于胃癌细胞、固有腺体和浸润炎性细胞的细胞核内。通过与临床病理学指标比较显示，CXCL16的核表达与浸润深度、淋巴管浸润和 UICC 分期密切相关，并提示良好预后。结论：CXCL16可能参与胃癌的发生发展，其组织中的表达水平能够提示胃癌细胞的生物学行为和胃癌患者的预后。%To clarify the role and clinicopathologic significance of CXC chemokine ligand 16 and che-mokine(C - X - C motif)receptor 6 expression in gastric carcinoma. Methods:CXCL16 expression of tissue microar-rays containing gastric carcinoma and nonneoplastic mucosa was examined by immunohistochemistry and reverse tran-scription - polymerase chain reaction. Results:Among 28 pairs of gastric carcinoma and normal tissues,there was higher CXC chemokine ligand 16 expression in carcinoma than in adjacent mucosa(P ﹤ 0. 05). Nuclear CXC chemo-kine ligand 16 expression was correlated inversely with the depth of invasion,lymphatic invasion,stage,and favorable prognosis. Patients with lymphatic invasion or mixed - type carcinoma showed lower CXC chemokine ligand 16 con-centrations than those with no lymphatic invasion or with diffuse - type carcinoma(P ﹤ 0. 05). Conclusion:Aberrant expression of CXC chemokine ligand 16 might be involved in gastric carcinogenesis. The expression and serum con-centration of CXC chemokine ligand 16 could indicate the aggressiveness and prognosis of gastric carcinomas.