血清高迁移率族蛋白 B1在非小细胞肺癌中的表达及其临床意义

摘要

To investigate the expression of HMGB1 in non - small cell lung cancer and to analyze the correlation between HMGB1 and clinicopathologic outcomes. Methods:The serum HMGB1 levels in 30 patients with non - smal cell lung cancer and 15 healthy controls were determined by real time PCR and Western - blot and analys-ize their relationship with clinical pathology parameter. Results:The expression of HMGB1 mRNA and protein was significantly higher in non - small cell lung cancer group than in healthy control group. Furthermore,expression of HMGB1 protein in non - smal cell lung cancer were related to TNM staing(P ﹤ 0. 05)and lung cancer cell differenti-ation(P ﹤ 0. 05),but not to smoking,sex and histological type(P ﹥ 0. 05). Multiple comparisons showed that there were different expressions of HMGB1 with different differentiation,(0. 43 ± 0. 10),(0. 75 ± 0. 31),(0. 98 ± 0. 24) (P ﹤ 0. 05). HMGB1 protein of stage Ⅰ - Ⅱ(0. 62 ± 0. 29)were significantly higher than those of stage Ⅲ - Ⅳ(0. 85 ± 0. 32)(P ﹤ 0. 05). Conclusion:HMGB1 may be a useful clinical marker for evaluating the NSCLC progres-sion and is of potential prognostic value.%目的：探讨血清高迁移率族蛋白 B1（high mobility group box 1，HMGB1）在非小细胞肺癌（NSCLC）中的表达及其与肺癌临床、病理之间的关系和意义。方法：应用 RT - PCR 及 Western - blot 印记法检测30例经病理证实的非小细胞肺癌患者及15名健康对照组血清 HMGB1水平，并分析与临床病理资料的关系。结果：非小细胞肺癌组血清 HMGB1 mRNA 及蛋白水平明显高于健康对照组。在非小细胞肺癌中，HMGB1的表达与 TNM 分期和细胞分化程度密切相关（P 均﹤0.05），但与吸烟、性别、病理类型无关（P 均﹥0.05）。高、中、低分化肺癌中 HMGB1蛋白的表达量分别为（0.43±0.10）、（0.75±0.31）、（0.98±0.24），三者间差异具有统计学意义（P ﹤0.05）。Ⅰ、Ⅱ期病例中 HMGB1蛋白的表达量为（0.62±0.29），明显低于Ⅲ、Ⅳ期（0.85±0.32）（P ﹤0.05）。结论：HMGB1在非小细胞肺癌中高表达，与肺癌 TNM 分期及细胞分化有关，可能作为判断非小细胞肺癌进展和预后判断的一种有效指标。