GRP78和TopoⅡ在胃腺癌组织中的表达及意义

摘要

Objective:To investigate the expression of GRP78 and TopoⅡin human gastric adenocarcinoma,and to explore the role in gastric cancer drug resistance. Methods:The expressions of GRP78 and TopoⅡ were detected in 60 cases of gastric adenocarcinoma and 20 cases of tumor adjacent tissue by immunohistochemistry. Results:The ex-pression of GRP78 in most tumor adjacent tissues was weakly positive,but in most gastric adenocarcinoma was strong-ly positive. GRP78 was also found in vascular endothelial cells. The expression was closely related with the size,de-gree of malignance,depth invasion and lymph node metastasis(P<0. 01),but not related with gender and age. The expression of TopoⅡin high differentiation adenocarcinoma was higher than low differentiated adenocarcinoma( P<0. 05),but there was no difference between gender,age,tumor size,depth infiltration,and lymph node metastasis(P>0. 05). The expression of GRP78 and TopoⅡ had no significant correlation(P>0. 05). Conclusion:The overex-pression of GRP78 in gastric carcinoma may have effects on drug resistance,whether GRP78 can decrease the expres-sion of TopoⅡ need further research.%目的：研究GRP78和TopoⅡ蛋白在胃腺癌组织中的表达及两者的相关性，探讨两者在胃腺癌耐药性中的作用。方法：运用免疫组化法检测60例胃腺癌和20例癌旁组织中GRP78和TopoⅡ的表达。结果：GRP78在癌旁组织呈弱阳性表达，在胃腺癌组织中呈中、强阳性表达，且在腺癌组织中的血管内皮细胞也有表达。GRP78在腺癌中的表达与性别、年龄等无关，而与肿瘤大小、分化程度、浸润深度、有无淋巴结转移密切相关（ P＜0.01）。TopoⅡ在高中分化腺癌中表达高于低分化腺癌（ P＜0.05），而与性别、年龄、肿瘤大小、浸润深度、有无淋巴结转移均无关（P ＞0.05）。GRP78与 TopoⅡ的表达无明显相关性（P ＞0.05）。结论：GRP78在胃腺癌中高表达，对胃癌耐药性有一定影响，GRP78在胃腺癌中是否降低TopoⅡ的表达有待进一步研究。