CD44基因在结肠癌组织中的表达及其意义

摘要

Objective:To determine the expression of CD44 gene mRNA in colorectal cancer. Methods:Twenty five colorectal cancer tissues and matched normal tissues were collected by surgery,total RNA extraction and reverse transcription into cDNA. The levels of CD44 mRNA were detected by using Real-time PCR. Results:The levels of CD44 mRNA were higher in cancer tissues than that in normal tissues(P<0. 05). High CD44 expression was not as-sociated with sex(P=0. 804),age(P=0. 592),differentiation(P=0. 443),lymphatic invasion(P=0. 525),venous invasion(P=0. 592),tumor size(P=0. 592)and pN category(P=0. 824). CD44 expression was associated with sur-vival rate of the patients with colorectal cancer by using Kaplan-meier survival curve analysis(P=0. 041). Conclu-sion:Abnormal high CD44 mRNA expression was observed in colorectal cancer tissues,which suggest that there was association between CD44 and the development of colorectal cancer.%目的：检测CD44基因mRNA在结肠癌组织中的表达。方法：收集25例明确诊断的结肠癌组织及其相应癌旁组织，提取总RNA并逆转录成cDNA。采用Real-time PCR方法检测CD44基因在结肠癌组织和癌旁组织中的 mRNA水平。结果：CD44基因在结肠癌组织中的含量明显高于癌旁组织（ P ＜0.05）。CD44基因与患者的性别（ P ＝0.804）、年龄（P＝0.592）、分化（P＝0.443）、淋巴侵袭（P＝0.525）、静脉侵袭（ P＝0.592）、肿瘤大小（ P＝0.592）和病理分期（ P＝0.824）等因素无关。Kaplan-meier生存曲线分析显示CD44基因与结肠癌患者预后具有显著相关性（P＝0.041）。结论：CD44基因mRNA在结肠癌组织中表达异常升高，提示CD44可能与结肠癌的发生、发展有密切关系。