Bcl-2、p53和ki-67在甲状腺癌中的表达及其临床意义的研究

摘要

Objective:To investigate expressions of Bcl-2,p53 and ki-67 in thyroid carcinoma and their clinical significance. Methods:SP immunohistochemical method was used to assess the expressions Bcl-2,p53 and ki-67 in thyroid carcinoma( TC group),thyroid adenoma( TA group)and normal thyroid tissues( control group). Results:Bcl-2 was expressed in 57. 5% of the thyroid carcinoma,20. 0% of the thyroid adenoma,and non in normal thyroid tissues. The positive expression rate of Bcl-2 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma(P<0. 05). The positive expression rate of p53 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma(P<0. 05). The positive expression rate of ki-67 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma(P<0. 05). In thyroid carcinoma,undifferentiated carcinoma,follicular carci-noma,carcinoma with positive lymph nodes,and tumors with stage Ⅲ andⅣ disease had significantly higher expres-sion of Bcl-2. Conclusion:Bcl-2,p53 and ki-67 play an important role in the development of thyroid carcinoma and the overexpression of them might be a possible prognostic factor of thyroid cancer.%目的：研究甲状腺癌组织中Bcl-2、p53和ki-67的表达及其临床意义。方法：采用免疫组织化学SP法检测甲状腺癌组织、甲状腺腺瘤和正常甲状腺中Bcl-2、p53和ki-67的表达情况。结果：Bcl-2、p53和ki-67的阳性表达见于甲状腺癌组织和甲状腺腺瘤中，正常甲状腺组织中未见三者的阳性表达。甲状腺癌组织中Bcl-2的阳性表达率明显高于腺瘤组织（ P＜0.05），甲状腺癌组织中p53的阳性表达率明显高于腺瘤组织（P＜0.05），ki-67在甲状腺癌中的阳性表达率为71.3％，高于甲状腺腺瘤10.0％（P＜0.05）。甲状腺未分化癌和滤泡状癌组织中Bcl-2的阳性率明显增高。有淋巴结转移和临床Ⅲ、Ⅳ期病例中，Bcl-2的阳性率明显增高。结论：Bcl-2、p53和ki-67在甲状腺癌发生发展中起重要作用，Bcl-2、p53和ki-67的表达可作为甲状腺癌预后的指标。