ING4诱导甲状腺癌SW579细胞S早期阻滞的机制研究

摘要

Objective:To study the effects and mechanisms of ING4 on cell cycle of thyroid cancer cell SW579. Methods:The Proliferative ratio of SW579 cells after treated with ING4 was detected by soft agarose assay. We detec-ted the changes of cell cycle after treated with ING4 by PI staining and Cldu/ Idu double staining. The cell cycle relat-ed Proteins were analyzed by Western blot. Results:ING4 could inhibit the Proliferation and induced S Phase early stage arrest in SW579 cells. The levels of cell cycle related Proteins,P53,PhosPho - S345 - Chk1 and PhosPho - T68- Chk2,were higher in treated cells than untreated ones. Conclusion:ING4 could induce S early stage arrest in SW579 cell,uP - regulate P53 exPression and cell cycle related Proteins. eowever,the relationshiP among ING4,P53 and cell cycle related Proteins by activating P53 exPression was needed further studies.%目的：检测 ING4对甲状腺癌 SW579细胞周期的影响及其机制。方法：利用软琼脂克隆形成实验检测细胞增殖，并利用 PI 染色和 Cldu/ Idu 双染检测细胞周期变化。Western blot 技术检测周期相关蛋白的变化。结果：ING4可以明显抑制 SW579细胞增殖，并且导致其 S 早期阻滞。ING4处理后细胞 P53表达明显升高，同时 PhosPho - S345- Chk1和 PhosPho - T68- Chk2等细胞周期相关蛋白表达水平升高。结论：ING4可导致 SW579细胞出现 S 早期阻滞，P53表达明显升高，细胞周期相关蛋白表达水平升高，但是三者关系有待进一步研究。