兔脂肪干细胞增强脂肪移植效果的实验研究

摘要

Objective:To establish an animal model for the injectable transPlantation fat tissue transPlantation and to investigate the morPhological changes of rejection after ear transPlantation in rabbit. Methods:All 24 healthy New Zealand white rabbits were divided into four grouPs randomly. The autologous adiPose granule(AG)were imPlanted in the ears of the rabbits as the exPeriment grouP A(n = 6). The autologous adiPose granule(AG)combined with Platelet - rich fibrin(PRF)were imPlanted in ears as the exPeriment grouP B(n = 6). The autologous adiPose gran-ule(AG)combined with autologous adiPose - derived stem cells(ADSCs)were imPlanted in ears as the exPeriment grouP C(n = 6). The autologous adiPose granule(AG)combined with Platelet - rich fibrin(PRF)and autologous adiPose - derived stem cells(ADSCs)were imPlanted in the ears were the control grouP D(n = 6). At month 1,3 and 6 after transPlantation,the survival rates of transPlanted ears,eE staining,rabbit ears light transmission exPeri-ments were Performed. Results:At month 1,3 and 6 after transPlantation,the survival rates of transPlanted ears,eE staining,rabbit ears light transmission exPeriments,the differences of the grouP D and grouP A,B,C were statistical significant(P ﹤ 0. 05). Conclusion:The adiPose granule(AG)combined with Platelet - rich fibrin(PRF)and adi-Pose - derived stem cells(ADSCs)can imProve the survival rate of transPlanted fat tissue and Provide exPerimental basis for clinical fat transPlantation.%目的：建立兔耳脂肪移植模型，观察兔脂肪干细胞（ adiPose - derived stem cells，ADSCs）复合脂肪颗粒（adiPose granule，AG）和富血小板纤维蛋白（Platelet - rich fibrin，PRF）移植后的形态学变化，为临床脂肪干细胞移植提供实验依据。方法：以健康新西兰家兔为实验动物，共取24只，随机分成4组（n ＝6）：A 组移植物为 AG；B 组 AG + PRF；C 组 AG + ADSCs；D 组 AG + PRF + ADSCs。在术后1、3、6个月，用 B 超、游标卡尺测量移植脂肪后的体积变化。透射光活体观察各时间点新生血管的形成及 eE 染色分析其形态学改变。结果：术后1、3、6个月在脂肪存活率、免疫组化、透光观察等方面 D 组与 A、B、C 三组比较均有显著性差异（P﹤0.05）。结论：ADSCs 复合 PRF、AG 能够显著提高移植脂肪组织的成活率，可为临床脂肪干细胞移植提供实验依据。