黄连素对肺腺癌 A549细胞增殖、迁移和侵袭的影响

摘要

Objective:To study the effect of Berberine on Proliferation,migration and invasion of A549 cells. Methods:The Proliferation and aPoPtosis of A549 cells were determined by MTT and FACS resPectively. The rate of A549 cell migration was observed by Transwell assay. The exPression of E - cadherin,Vimentin,Slug,Snail in A549 cells effected by Berberine were assessed by Western blot. Results:The Proliferation of A549 cell could be significant-ly inhibited within limited duration and dosage(P ﹤ 0. 05). Also exPression of Vimentin,Snail and Slug of gene were decreased but E - cadherin increased after Berberine treatment(P ﹤ 0. 05). Conclusion:Berberine can inhibit the mi-gration abilities of A549 cells that might be related to down - regulating the exPression of Vimentin,Snail and uP -regulating the exPression of E - cadherin.%目的：研究黄连素（BBR）对 A549细胞增殖、迁移和侵袭的影响，初步探讨 BBR 抑制 A549细胞转移的机制。方法：用不同浓度的 BBR 作用于 A549细胞，MTT 法测定 A549细胞增殖水平，流式细胞仪检测 A549细胞的凋亡，Transwell 实验测定 A549细胞的迁移和侵袭能力，Western blot 测定 A549细胞中与 EMT 相关的蛋白 E - cadherin、Vimentin、Slug、Snail 的表达。结果：BBR 以浓度及时间依赖的方式抑制 A549细胞的增殖；流式细胞仪检测发现 BBR 作用于 A549细胞后凋亡率呈剂量依赖性增加；Western blot 显示 BBR 作用 A549细胞后，E - cadherin 表达升高，Vimentin、Slug、Snail 表达降低，Transwell 实验显示 BBR 作用于 A549细胞后，能够抑制其迁移和侵袭。结论：BBR 能抑制 A549细胞增殖，诱导其凋亡，通过促进 E - cadherin 表达和抑制Vimentin、Slug、Snail 的表达来抑制 EMT 的发生，从而抑制了 A549细胞的迁移和侵袭。