BP1基因对肾透明细胞癌细胞株 RLC －310增殖、克隆形成及迁移能力的影响

摘要

目的：研究 BP1基因对肾透明细胞癌细胞株 RLC －310细胞增殖、克隆形成能力及迁移能力的影响。方法：将前期构建的质粒 pEGFP －N1－BP1转染入 RLC －310细胞系，用 Real －time PCR 和 Western blot检测 BP1 mRNA 与蛋白的表达情况；通过 MTT、平板克隆实验及 Transwell 实验检测 pEGFP －N1－BP1对 RLC－310细胞的作用。结果：转染了 pEGFP －N1－BP1的 RLC －310细胞中 BP1基因／蛋白的表达明显上调；转染组较空白对照组细胞增殖能力明显增加（P ＜0．05），克隆能力及迁移能力增强（P ＜0．05）。结论：BP1基因能够增强 RLC －310细胞的增殖、克隆形成能力及迁移能力。%Objective:To explore the effects of pEGFP -N1 -BP1 on proliferation,clone formation and migration of RLC -310 cells.Methods:The pEGFP -N1 -BP1 was transfected into RLC -310 cells.The expression of BP1 gene was detected by Real -time PCR and Western blot.The effects of proliferation,clone formation and migration of RLC -310 cells were examined by MTT,clone formation assay and Transwell assay respectively.Results:The RLC -310 cells transfected with pEGFP -N1 -BP1 plasmid expressed a higher level of Twist protein steadily.The capabilities of proliferation,clone formation and migration of RLC -310 cells increased significantly compared with blank control group(P <0.05).Conclusion:BP1 gene can enhance the abilities of proliferation,clone formation and migrationof RLC -310 cells.