乳腺疾病患者血清中IFN-γ的检测及临床意义

摘要

目的：检测乳腺疾病患者及对照组患者血清中IFN-γ的水平，分析其临床意义。方法：收集2014年3月至2015年3月我院转移性乳腺癌患者30例、局部乳腺癌患者35例、乳腺不典型增生患者29例、乳腺纤维瘤患者31例、正常人群30例。应用酶联免疫吸附试验（ ELISA法）定量检测IFN-γ在不同组织血清中的水平。比较乳腺癌组与其他各组间血清IFN-γ水平的差异，同时分析乳腺癌组血清中IFN-γ水平与临床病理特征间的关系，探讨检测IFN-γ的临床意义。结果：转移性乳腺癌患者血清中IFN-γ水平明显高于局部乳腺癌组、乳腺不典型增生组、乳腺纤维瘤组及正常人群组，经统计学分析差异均具有统计学意义（F=142.48，P﹤0.05）。乳腺不典型增生组、乳腺纤维瘤组及正常人群组血清中IFN-γ的组间表达差异无统计学意义（ P﹥0.05）。乳腺癌患者中IFN-γ的表达水平与年龄和肿瘤大小无关（ P﹥0.05），而与TNM分期、组织学分级、淋巴结状态及脉管浸润有关，差异具有统计学意义（ P﹤0.05）。结论：IFN-γ血清水平与乳腺癌发生及侵袭转移相关，可作为一个潜在的乳腺癌血清标志物。%Objective:To detect the level of IFN-γin breast disease patients and control group,analyze its clini-cal significance. Methods:Collect 30 metastatic breast cancer patientsˊserum,35 patientsˊserum of localized breast cancer,29 patientsˊserum of atypical hyperplasia of the breast,31 mammary gland fibroma patientsˊserum and 30 nor-mal peopleˊs serum. Use the ELISA method to quantitatively detect the level of IFN-γ. Results:The level of IFN-γin metastatic breast cancer patientsˊserum was apparently higher than localized breast cancer group,atypical hyperpla-sia of the breast group,mammary gland fibroma group and normal population group(F=142. 48,P﹤0. 05). The lev-els of IFN-γ in atypical hyperplasia of the breast group,mammary gland fibroma group and normal population group had no difference(P﹥0. 05). There was no relationship among age,tumor size and the expression level of IFN-γin breast cancer patients(P﹥0. 05),but among TNM staging,histological grade,node status and vascular lymphatic in-vasion,the differences were significant(P ﹤0. 05). Conclusion:IFN -γ is related to the genesis and invasion of breast cancer,it is a potential biomarKer for breast cancer diagnosis.