PAS改良染色法及其在病理诊断中的应用

摘要

目的：改良高碘酸-无色品红染色方法（ PAS），改善PAS染色结果，为临床病理诊断提供可靠依据。方法：脱蜡至水的病理切片。选择2%高碘酸钠作为氧化剂，严格控制氧化剂20分钟作用时间，使用自行配制的Schiff试剂染色孵育5分钟，苏木素衬染，观察染色结果。并将300例含头颈、肺、肾脏、肝脏及胃标本的染色结果与传统的PAS染色法染色结果相互比较。结果：改良后PAS染色方法的PAS阳性物质（黏液、糖原等）颜色鲜艳，特异性强。结论：影响PAS染色的因素很多，使用2%高碘酸钠氧化液，严格控制染色温度和时间，可有效改善PAS染色结果。%Objective:To refine the periodic acid schiff( PAS)histochemical staining method and to improve quality of staining. Methods:A modified special Schiff reagent was used,then sodium periodate as the oxidizing agent with a reaction time of 20 min. We compared the new reagent with traditional PAS staining in 300 specimens including head & necK,lung,Kidney,liver and stomach. Results:Mucus and glycogen was stained red,the nuclei was stained purple. The effect of the stain was excellent in the improved method. Conclusion:Raised concentration of oxidizing so-lution,a proper dyeing duration and coloration temperature,which improve the PAS staining of glycogen.