富含半胱氨酸的酸性分泌蛋白在喉癌和下咽癌间质组织中表达及功能

摘要

Objective:To explore the contribution of SPARC for head and neck squamous carcinoma,investigate protein expression and function of SPARC in laryngeal and hypopharyngeal carcinoma. Methods:Immunohistochemi-cal staining was used to detect the expression of SPARC in primary tumor,recombinant human SPARC was used to de-termine the effect of exogenous SPARC on growth and migration of tumor cell lines. Results:Immunohistochemical staining showed that stromal SPARC was down-regulated in 46. 3%(19/41)of laryngeal carcinoma and 60. 0%(6/10)of hypopharyngeal carcinoma. Exogenous SPARC suppressed the growth and migration of tumor cell lines. Conclu-sion:Down-regulated expression of stromal SPARC promotes the proliferation of laryngeal and hypopharyngeal carci-noma cells.%目的：通过研究富含半胱氨酸的酸性分泌蛋白（ SPARC）在喉癌及下咽癌的蛋白表达与相关功能，探讨SPARC对头颈鳞癌发展所起的作用。方法：免疫组织化学染色检测SPARC在51例肿瘤组织中的表达，观察外源性SPARC对体外细胞系生长及迁移的影响。结果：免疫组化染色显示SPARC在46.3％（19/41）喉癌及60.0％（6/10）下咽癌的间质组织表达下调，外源性SPARC抑制头颈鳞癌细胞株的增殖及迁移。结论：肿瘤间质SPARC表达下调促进了喉癌及下咽癌细胞的增殖。