Sema3 A在胃癌中的表达及意义

摘要

目的：探讨Sema3A在胃癌中的表达及意义。方法：利用酶联免疫吸附法（ ELISA）检测正常健康人及胃癌患者血清中Sema3A表达的差异性。应用实时荧光定量聚合酶链反应（ Real time PCR）检测胃癌细胞系、人正常胃黏膜细胞中Sema3A mRNA的表达差异。应用免疫印迹法（ Western Blot）检测胃癌细胞系、人正常胃黏膜细胞中Sema3A 蛋白的表达差异。结果：与正常健康对照者相比，Sema3A在胃癌患者血清中表达下降（P＜0.001），且与胃癌患者的年龄、肿瘤分化程度相关（P＜0.05）。人转移潜能胃癌细胞株MKN-28M、MKN-28NM中Sema3A mRNA和蛋白表达量明显低于人正常胃黏膜细胞（ P＜0.05），且在高转移潜能MKN-28M细胞中表达量最低（ P＜0.05）。结论：与正常对照组相比，Sema3A在胃癌患者血清、人胃癌细胞株中均表达下降，且在胃癌高转移潜能MKN-28M细胞中表达最低，提示Sema3A在胃癌的发生发展中具有重要作用，可能扮演着抑癌基因的作用。%Objective:To investigate the exPression of SemaPhorin 3A in gastric carcinoma and its clinical signifi-cance. Methods:The concentration of Sema3A in the serum was detected by the enzyme-linked immune sorbent as-say(ELISA)in 104 Patients with gastric cancer,20 healthy controls. Sema3A was detected in high and low metastatic gastric cancer cell lines through RT-qPCR and Western Blot method. Results:Sema3A exPression in gastric cancer Patients serum decreased(P<0. 001)and was associated with stomach cancer Patients age and tumor differentiation degree(P<0. 05). Gastric cancer transferred cell lines MKN-28M,MKN-28NM in Sema3A mRNA and Protein exPression was significantly less than the normal human umbilical vein endothelial cells(P<0. 01),but not in SGC-7901 cell lines(P>0. 05). In addition,the exPressing of Sema3A was quantity minimum in MKN-28M cells. Con-clusion:The exPression of Sema3A declined in gastric cancer Patients serum and gastric cancer cell lines,which are metastatic Potential. In addition,the exPression of Sema3A was quantity minimum in high metastasis Potential gastric cancer MKN-28M cells. Sema3A,as a tumor suPPressor,Plays an imPortant role in inhibiting gastric cancer develoP-ment.