长链非编码RNA MEG3抑制人胃癌SGC-7901细胞增殖的研究

摘要

目的：探讨长链非编码RNA MEG3在临床胃癌组织及细胞中的表达情况，以及过表达MEG3对人胃癌细胞增殖能力的影响。方法：采用实时定量PCR检测人胃癌组织及细胞系中MEG3的表达，并结合病理资料分析胃癌组织MEG3表达与临床病理特征之间的关系。采用四甲基偶氮唑蓝（ MTT）法和克隆形成实验检测过表达MEG3前后胃癌细胞增殖的变化。结果：相对于正常胃组织及细胞，在胃癌组织和细胞中MEG3的表达水平明显下调。胃癌组织中MEG3表达与组织学分级、肿瘤浸润深度和TNM分期相关，但与年龄、性别及区域淋巴结转移无关。在SGC-7901细胞中转染MEG3过表达质粒后能显著上调MEG3的表达水平。过表达MEG3能明显抑制胃癌细胞的增殖能力。结论：MEG3对胃癌细胞增殖的调控至关重要，过表达MEG3能明显抑制胃癌细胞的增殖能力。提示MEG3的表达下调可能在胃癌的发生、发展中起重要作用。%Objective:To investigate the expression level of MEG3 in gastric cancer tissues and cell lines,and study the effect of overexpression of MEG3 expression on gastric cancer cells proliferation. Methods:Real -time quantitative PCR was performed to detect the relative expression of MEG3 in gastric cancer cell lines and tissues. And we explored the correlation of MEG3 expression level with the clinic-pathological factors in gastric cancer patients. MTT and colony formation assays were conducted to detect the effect of MEG3 on gastric cancer cells proliferation. Results:MEG3 expression was lower both in gastric cancer samples and cell lines compared with their corresponding normal tissues and cell lines. The results demonstrated that the expression of MEG3 was correlated with histological grade,depth of tumor invasion and TNM stage,but not with age,gender and lymph node metastasis. Overexpression of MEG3 could significantly inhibit SGC-7901 cells proliferation. Conclusion:Overexpression of MEG3 can affect the proliferation of gastric cancer cell. It is possible that MEG3 may play a key role in the occurrence and development of gastric cancer.