VEGF和pRb2/p130、CyclinD1在子宫内膜癌中的表达及意义

摘要

Objective:To investigate the expressions of pRb2/p130,CyclinD1 protein and VEGF,their correlation and relationship with the pathological clinical features of normal endometrial tissue,endometrial hyperplasia,atypical endometrial hyperplasia and human endometrial carcinoma. Methods:Thirty normal endometrial tissue,30 endometrial hyperplasia,40 atypical endometrial hyperplasia and 68 endometrial carcinomas were collected. Immunohistochemistri-cal methods were used to detect their pRb2/p130,CycinD1 protein and VEGF expressions. Results:In the atypical endometrial hyperplasia,the expressions of VEGF were significantly higher than that of normal(p﹤0. 05). VEGF and CyclinD1 expression were positive correlated. In the endometrial carcinomas,the expression of VEGF was associated with tumor grade and ER. The expressions of VEGF were significantly higher than that of endometrial hyperplasia( p﹤0. 05). There was negative correlation between the expressions of pRb2/p130 protein and that of VEGF(r= -0. 243, p=0. 045). There was positive correlation between the expressions of CyclinD1 protein,ER and that of VEGF(r=0. 337,p=0. 005;r =4. 628,p =0. 031,respectively). Conclusion:The abnormal expression of pRb2/p130,Cy-clinD1 protein and VEGF in the atypical endometrial hyperplasia and that of endometrial carcinomas,combined to-gether,may be as screening index of precancerous lesions and endometrial cancer aggressiveness. Estrogen-receptor may be regulate production of VEGF.%目的：探讨pRb2/p130、CyclinD1蛋白和VEGF在正常子宫内膜、子宫内膜增殖症、非典型子宫内膜增殖症和子宫内膜癌中的表达、相关性及与临床病理特征的关系。方法：免疫组织化学EnVision二步法检测pRb2/p130、CyclinD1蛋白和VEGF在30例正常增生期子宫内膜、30例子宫内膜增殖症、40例非典型子宫内膜增殖症和68例子宫内膜癌中的表达。结果：在非典型子宫内膜增殖症中，VEGF的表达显著高于正常子宫内膜（ p﹤0.05），与CyclinD1呈正相关（ p＝0.013）。子宫内膜癌中，VEGF的表达分别与组织学分级、雌激素受体（ER）相关。VEGF 的表达显著高于子宫内膜增殖症（p﹤0.05），VEGF与pRb2/p130呈负相关（r＝-0.243，p＝0.045），分别与CyclinD1蛋白、ER呈正相关（r＝0.337，p＝0.005；r＝4.628，p＝0.031）。结论：pRb2/p130、CyclinD1蛋白和VEGF在非典型子宫内膜增殖症和子宫内膜癌中的异常表达，结合在一起可能成为癌前病变和子宫内膜癌侵袭的筛查指标。子宫内膜癌中，ER可能调控着VEGF的产生。