加温苏木素染液在术中冰冻染色中的应用

摘要

目的：探讨室温较低时最佳的快速苏木素染色温度，以改善术中切片染色质量，提高诊断准确性。方法：选取不同新鲜活体组织，包埋剂处理，经快速冰冻切片机切片，不同梯度温度苏木素染液染色，伊红快速染色。结果：当室温较低时，苏木素染液温度在35℃染色质量最好，染色鲜艳，镜下组织结构细胞形态清晰，细胞核浆对比清楚。结论：控制好染色液温度可辅助提高病理诊断的准确性。%Objective:To explore the best temperature of rapid hematoxylin staining,improving the dyeing effect of the frozen section and the diagnostic accuracy. Methods:Several kinds of fresh tissue were used for rapid hematoxylin staining in different temperature gradients. Results:When the room temperature drops,the optimal dyeing effect of he-matoxylin staining was obtained under 35℃ . Conclusion:Controlling temperature accurately can improve dyeing effect of frozen sections.