土槿皮乙酸对胰腺癌 AsPC -1细胞增殖和凋亡的影响

摘要

目的：观察土槿皮乙酸（pseudolaric acid B，PAB）对胰腺癌 AsPC -1细胞增殖和凋亡的影响。方法：体外培养胰腺癌 AsPC -1细胞，用不同浓度 PAB 作用不同时间后，MTT 法检测 PAB 对 AsPC -1细胞增殖的影响，流式细胞仪检测细胞凋亡率。结果：MTT 结果表明 PAB 以时间和剂量依赖的方式抑制 AsPC -1细胞生长，流式结果显示8.0μmol/ L PAB 处理 AsPC -124小时后，凋亡率由对照组的8.19%升高至30.86%，差异有统计学意义（P <0.05），表明 PAB 可诱导胰腺癌细胞凋亡，呈剂量依赖性。结论：土槿皮乙酸以时间和剂量依赖的方式抑制胰腺癌 AsPC -1细胞增殖并诱导其凋亡。%Objective:To examine the effects of pseudolaric acid B(PAB)on the proliferation and apoptosis of human pancreatic carcinoma cell line ASPC - 1. Methods:ASPC - 1 cells cultured in vitro were treated with various concentrations(0. 5,1. 0,2. 0,4. 0,8. 0μmol/ L)of PAB at various intervals. Growth inhibition was analyzed by 3 -(4,5 - dimethylthiazol - 2 yl)- 2,5 - diphenyltetrazolium bromide(MTT)assay. Apoptotic cells were detected by flow cytometry. Results:Pseudolaric acid B inhibited the proliferation of ASPC - 1 cells in a time - and dose - de-pendent manner. The inhibition ratio was(4. 53 ± 3. 32)% after the cells were treated with 0. 5μmol/ L PAB for 24h, and the ratio was(52. 22 ± 1. 71)% after the cells were treated with 8. 0μmol/ L PAB for 72h. ASPC - 1 cells treated with 8. 0μmol/ L PAB for 24h showed significantly enhanced apoptosis as compared with the control cells(8. 19% vs 30. 86% ,P < 0. 05 ).Conclusion:PAB can dramatically suppress the ASPC -1 cell growth and induce cell apoptosis.