miR -22抑制食管鳞状细胞癌细胞侵袭和转移

摘要

目的：探讨微小 RNA -22（miR -22）对食管鳞状细胞癌细胞侵袭和转移的影响。方法：采用实时定量 RT - PCR 检测 miR -22在食管鳞状细胞癌细胞系中的表达。过表达 miR -22后应用 Transwell 小室、MTT增殖及划痕试验分析对食管鳞状细胞癌细胞侵袭和转移能力的影响。结果：在食管鳞状细胞癌细胞系中 miR-22的表达比正常对照明显降低。此外，过表达 miR -22可明显抑制食管鳞状细胞癌细胞系 Eca109和 TE-1细胞增殖、侵袭和转移能力。结论：miR -22作为肿瘤抑制小片段 RNA 可以抑制食管癌细胞的侵袭和转移。本研究有助于了解 miR -22在食管鳞状细胞癌中的功能，为食管鳞状细胞癌治疗提供新靶点。%Objective:To explore the effect of miR - 22 on the metastasis and invasion of esophageal squamous cell carcinoma(ESCC)cell line. Methods:The miR - 22 expression level was measured by real - time quantitative RT - PCR in ESCC cell lines. We performed the invasion assay,MTT proliferation assay and wound - healing assay to test the invasion and proliferation of ESCC cell after overexpression of miR - 22. Results:The expression of miR - 22 in ESCC cell lines were much lower than that in normal control. Moreover,transfection of miR - 22 expression plasmid could significantly inhibit the cell proliferation,migration and invasion in Eca109 and TE - 1 ESCC cell lines. Con-clusion:The tumor suppressor,miR - 22 could inhibit ESCC cell migration and invasion. Our findings contribute to the current understanding of the expression and function of miR - 22 in ESCC.