BRCA-1和 MycmRNA 联合检测在乳腺癌诊断中的价值

摘要

Objective:To evaluate combined detection of BRCA - 1 and Myc gene in the diagnosis of breast canc-er. Methods:We used FQ - PCR to detect BRCA - 1 and Myc mRNA expression in samples from 35 health women, 40 patients with benign breast disease and 96 patients with breast cancer. β2 - microglobin was used as internal con-trol. Results:No significant difference was found in BRCA - 1 and Myc/ β2 - microglobin between normal controls and benign breast disease group(P ﹥ 0. 05). BRCA - 1 / β2 - microglobin in breast cancer group was lower than those in normal controls and benign breast disease group(P ﹤ 0. 05). Myc/ β2 - microglobin in breast cancer group was higher than those in normal controls and benign breast disease group(P ﹤ 0. 05). No difference was found in β2 - microglo-bin among three groups(P ﹥ 0. 05). The positive number of BRCA - 1 and Myc in 96 patients with breast cancer were 46 and 41,the positive ratio were 47. 9% and 42. 7% . The positive number of combined detection of BRCA - 1 and Myc was 55,the positive ratio was 57. 3% . The sensitivity of combined detection of BRCA - 1 and Myc gene express was higher than that of single gene detection. Conclusion:FQ - PCR is a rapid,sensitive and specific method for quantitating BRCA - 1 and Myc. The combined detection can improve the sensitivity of diagnosis in breast cancer.%目的：荧光定量聚合酶链反应（FQ - PCR）法检测乳腺癌易感基因（breast cancer susceptibility gene 1， BRCA -1）和核内原癌基因（nuclear oncogene，Myc）表达水平，探讨其联合检测在乳腺癌诊断和治疗监测中的应用。方法：建立 FQ - PCR 法，并以β2-微球蛋白为内对照测定35例健康女性体检者、40例良性乳腺肿瘤和96例乳腺癌外周血中 BRCA -1 和 Myc 的表达量。结果：正常对照组、良性乳腺疾病和乳腺癌组 BRCA -1和 Myc 表达水平在正常对照组和良性乳腺疾病组间差异无显著性意义（ P ﹥0.05），BRCA -1 在乳腺癌组显著低于正常对照组和良性乳腺疾病组（P ﹤0.05），Myc 在乳腺癌组显著高于正常对照组和良性乳腺疾病组（P﹤0.05），β2-微球蛋白在三组间差异无显著性意义（P ﹥0.05）。96例乳腺癌患者中 BRCA -1 和 Myc 的阳性数分别为46和41例，阳性率分别为47.9％和42.7％；BRCA -1 和 Myc 联合检测的阳性数为55例，阳性率为57.3％，两者联合检测的灵敏度高于单个基因的检测。结论：FQ - PCR 技术是高度灵敏、高度特异的快速定量检测 BRCA -1 和 Myc 的方法，两者联合检测可提高对乳腺癌诊断的灵敏度。