噬菌体肽库在宫颈癌血清肿瘤标志物筛选中的应用

摘要

Objective:To find the new specific tumor marker for breast cancer through screening the phage display peptide library in the serum of patients with cervical cancer. Methods:Three rounds of subtract biopanning were car-ried out. ELISA was used to confirm the specificity of phages clones. Results:After three rounds of screening,the en-richment ratio was increased. The eluted were enrich to 100 fold. The phage with high specificity was found and con-firm in the serum of 50 cases of cervical cancer patients and normal people by ELISA. It have potential value for early diagnosis of cervical cancer. Conclusion:A high affinity dodecapeptide specific for the serum of cervical cancer was obtained through phage display library. It can be used for the next step in tumor marker of cervical cancer and early diagnosis of cervical cancer.%目的：用噬菌体随机12肽库对宫颈癌患者血清进行差异性筛选，筛选出能与宫颈癌患者血清特异性结合的肿瘤标志物。方法：应用噬菌体随机12肽库对宫颈癌患者和正常人血清进行三轮差异性筛选，用ELISA 法检测噬菌体克隆对宫颈癌患者血清结合的特异性。结果：经过三轮筛选，噬菌体富集率逐轮提高，提高近100倍。用 ELISA 法对50例宫颈癌患者血清和50例健康者血清检测验证，其中获得一株与宫颈癌患者血清特异结合较好的噬菌体，对宫颈癌的早期诊断具有潜在价值。结论：筛选出能与早期宫颈癌患者血清高亲和力特异结合的短肽，为研究宫颈癌肿瘤标志物及宫颈癌的早期诊断奠定了基础。