目的：荧光定量聚合酶链反应（ FQ-PCR ）技术检测乳腺癌易感基因1（ breast cancer susceptibility gene 1，BRCA1）、乳腺癌易感基因2的表达及其与临床病理特征之间的关系，探讨其在散在乳腺癌诊断中的应用。方法：采用FQ-PCR法，并以甘油醛-3-磷酸脱氢酶（ GAPDH）为内对照测定30名健康女性体检者、55例良性乳腺疾病患者、96例乳腺癌患者外周血中BRCA1和BRCA2的表达量。结果：乳腺癌患者BRCA1和BRCA2的mRNA显著低于健康女性体检者和良性乳腺疾病患者。健康女性体检者和良性乳腺疾病患者BRCA1和BRCA2的mRNA无显著性差异。GAPDH和BRCA1/BRCA2比值在三组间无显著性差异。乳腺癌BRCA2 mRNA显著高于 BRCA1。BRCA1和 BRCA2 mRNA 之间有微弱的相关性（ r =0.378，p <0.01）。BRCA2 mRNA和BRCA1/BRCA2比值与临床病理（ ER和PR、组织学分级）存在相关性。BRCA1基因表达与临床病理不存在相关性。结论：采用FQ-PCR检测BRCA1和BRCA2基因表达水平，可有效监测散在乳腺癌的诊断、疗效和转移。%Objective:To evaluate the clinical application of fluorescent quantification polymerase chain reaction ( FQ-PCR)to detect BRCA1 and BRCA2 in the diagnosis of sporadic breast cancer and the relationship of their ex-pression with clinicopathological factors. Methods:BRCA1 and BRCA2 mRNA expression in the serum of 96 patients with sporadic invasive ductal carcinoma were quantified by FQ-PCR. GAPDH was used as internal control. Results:BRCA1 and BRCA2 mRNA was significantly lower than those in health women and patients with benign breast dis-ease. There was no significant difference in BRCA1 and BRCA2 mRNA between health women and patients with be-nign breast disease. There was no significant difference in BRCA1/BRCA2 and GAPDH among three groups. BRCA2 mRNA was significantly higher than BRCA1. There was a weak but significant correlation between BRCA1 and BRCA2 mRNA(r=0. 378,p<0. 01). There was significant association between BRCA2 mRNA expression and clin-icopathological factors( ER and PR,histological grade). There was no correlation between BRCA1 mRNA expression and clinicopathological factors such as menstrual status,tumor size,lymph node status,estrogen and progesterone re-ceptor status,and histological grade. There was difference between BRCA1/2 and clinicopathological factors( ER and PR,histological grade). Conclusion:FQ-PCR is a rapid method for quantitating BRCA1 and BRCA2. It also gives objective evidence to the diagnosis,therapy,metastasis of breast cancer.
展开▼
