百合多糖联合金雀异黄素对人类乳腺癌细胞增殖的影响

摘要

目的：研究百合多糖（ LP-1）联合金雀异黄素对人类乳腺癌细胞增殖的影响。方法：培养MCF-7细胞，加入不同浓度的LP-1和金雀异黄素，应用MTT法检测百合多糖对MCF-7细胞增殖的影响；用流式细胞技术（ FCM）检测细胞周期。放射性配体结合法检测化合物与雌激素受体（ estrogenic receptor，ER）的亲和力。结果：LP-1对MCF-7细胞增殖和细胞周期没有显著影响，和高浓度金雀异黄素合用可以增强金雀异黄素对MCF-7细胞的抗增殖作用，并将细胞周期阻滞在G2期。LP-1对ERα和 ERβ均无亲和力，金雀异黄素对ERα和ERβ均有亲和力，IC50分别（3.51×10-5）mol/L 和（2.12×10-7）mol/L。结论：百合多糖与金雀异黄素合用可能通过不同途径影响MCF-7细胞增殖和凋亡，发挥协同作用。%Objective:To investigate the effects of lily polysaccharide(LP-1)combined genistein on the prolifera-tion of mammary cancer cells in vitro. Methods:Effects of LP-1 and genistein on the cell proliferation were tested in MCF-7 cell by MTT measurement. Cell cycle was examined by flow cytometry. Competitive estrogen-receptor bind-ing assay was used to investigate the affinity of xy2004 to ER. Results:LP-1 used alone can′t inhibit proliferation of MCF-7 cell,while proliferation of MCF-7 cell was inhibited when LP-1 and genistein used together. The cell cy-cle was arrested to G2 by using polysaccharide and genistein together. The relative binding affinity of genistein for ERαand ERβ showed an IC50 of(3. 51 × 10-5 )mol/L and(2. 12 × 10-7 )mol/L respectively. Conclusion:LP-1 and genistein have the synergistic antitumor action through the different response pathway.