KLF4在宫颈癌中的表达与临床意义

摘要

目的：探讨转录因子KLF4在宫颈癌中的表达及临床意义。方法：采用免疫组化SP法检测78例宫颈癌、14例宫颈原位癌、15例慢性宫颈炎及13例正常宫颈组织中KLF4蛋白的表达并分析其与临床病理指标间的关系。结果：KLF4在宫颈癌、宫颈原位癌、慢性宫颈炎及正常宫颈组织中的阳性表达率分别为26.9%，71.4%，73.3%和84.6%，在宫颈癌中的阳性表达较其它各组明显下降（ p<0.05）。KLF4在宫颈癌中的表达与组织分型无关（p>0.05），在不同细胞分化等级与临床分期间KLF4表达有明显差异（p<0.05），主要表达于分化较好的组织中，随着分化程度的降低及临床分期的进展KLF4阳性表达率呈下降趋势，在伴有淋巴结转移的宫颈癌组织中KLF4的阳性表达率较不伴转移者显著下降（ p<0.05）。结论：KLF4在宫颈癌中的表达与其进展呈负相关，在宫颈癌的发生发展过程中发挥抑癌基因作用，有望成为预测宫颈癌发生及判断患者预后的新的参考指标。%Objective:To investigate the expression and clinical significance of Kruppel-like factor 4 in cervical carcinoma. Methods:The expression of KLF4 protein in 78 cases of cervical carcinoma,14 cases of cervical cancer in situ,15 cases of chronic cervicitis and 13 cases of normal cervical tissues was examined by SP immunohistochemical staining and the significance of the expression with clinicopathologic parameters was analyzed. Results:The positive expression of KLF 4 in cervical cancer ,cervical cancer in situ ,chronic cervicitis and normal cervical tissues was 26. 9%,71. 4%,73. 3% and 84. 6% respectively. KLF4 expression in cervical cancer significantly decreased com-pared with other groups(p <0. 05). There was no difference between histological type(p >0. 05),while obvious difference was related to different cell differentiation and clinical classification(p<0. 05),the main KLF4 expression located in the well differentiated tissue,and with the progress of the lower differentiation and clinical staging KLF4 positive expression was declined. KLF4 in cervical cancer with lymph node metastasis had the positive expression rate dropped significantly comparing with no metastasis(p<0. 05). Conclusion:The expression of KLF4 in cervical canc-er was negatively correlated with tumor progress and it plays a role of tumor suppressor genes in the development of cervical cancer,which was expected to be a new marker to predict the occurrence and the prognosis of patients with cervical cancer.