Heparanase、COX-2在肾癌中的表达和肿瘤血管生成的关系

摘要

Objective:To investigate the expression parterns of Heparanase and COX-2 protein in renal cell car-cinoma and their correlations with tumor progression and angiogenesis. Methods:The expressions of Heparanase and COX-2 in 54 renal cell carcinoma and 20 normal renal tissues were assessed by immunohistochemistry and the corre-lation with clinicopathologic features and microvessel density. Results:The expression of Heparanase and COX -2 protein significantly increased in renal cell carcinoma compared with that in normal renal tissues(p<0. 05). The ex-pression of Heparanase protein was positively correlated to the clinical stages,tumor diameter and distant metastasis of RCC(p<0. 05). There were no associations between the expression of Heparanase and age,gender and differentiation (p>0. 05). The expression of COX-2 protein was positively correlated to the clinical stage,differentiation and dis-tant metastasis of RCC(p<0. 05). There were no associations between the expression of COX-2 protein and tumor diameter,age and gender. RCC specimens had higher MVD than normal renal tissues(p<0. 05). Patients who showed high MVD was significantly associated with Heparanase and COX-2 positive tumors(p<0. 05). Heparanase and COX-2 positive tumors had a significantly higher MVD compared with negative tumors(p<0. 05). Expression of Heparanase was significantly correlated with that of COX-2(p<0. 05). Conclusion:Heparanase and COX-2 may be involved in malignancy of RCC through promoting tumor angiogenesis. It may be prognostic factors and therapeutic target of RCC.%目的：探讨Heparanase、COX-2在肾癌中表达的相关性及与肿瘤血管生成的关系及意义。方法：采用免疫组化PV6000法检测54例肾癌和20例正常肾脏组织中Hpa、COX-2蛋白的表达，以CD34单克隆抗体进行微血管内皮染色，计算微血管密度，分析Hpa、COX-2在肾癌中表达的相关性以及与临床病理特征及微血管密度的关系。结果：Hpa、COX-2在肾癌中的表达均较正常肾脏组织明显增高（ p<0.05）。Hpa的表达与肾癌的临床分期、肿瘤大小、远处转移有关（ p<0.05），而与患者年龄、性别、肿瘤的分化程度无相关性（ p