COX-2、TTF-1及p63蛋白在非小细胞肺癌中的表达及临床意义

摘要

Objective:To study the expressions of cyclooxygenase-2( COX-2 ),thyroid transcription factor-1 (TTF-1)and p63 protein in non-small cell lung cancer(NSCLC)as well as the relationship between their expres-sions and pathological type,clinical TNM stage and lymph node metastasis. Methods:The expression level of COX-2,TTF-1 and p63 protein were detected by immunohistochemistry in postoperative pathological tissues of 156 NSCLC patients. Results:In NSCLC patients tissues,the positive expression rates of COX-2,TTF-1 and p63 protein were 94. 5%(137/145),61. 0%(72/118),53. 5%(54/101),respectively. COX-2 expression was no significant differ-ence between adenocarcinoma and squamous carcinoma(p>0. 05),while the positive expression rates of TTF-1 and p63 protein were different(p<0. 001). The expression rates of COX-2 and p63 protein were not associated with NSCLC TNM clinical stage and lymph node metastasis(p>0. 05). In NSCLC patients with stage Ⅲ-Ⅳ or lymphatic metastasis,the positive expression rate of TTF-1 was significantly higher than that in stage Ⅰ -Ⅱ or without lymphatic metastasis patients(p<0. 01). Conclusion:The expression of TTF-1 and p63 protein might be effective biomarkers of adenocarcinoma and squamous carcinoma in NSCLC patients. The positive expression of TTF-1 might be related to infiltration,invasion and metastasis of lung cancer.%目的：研究环氧化酶-2（ COX-2）、甲状腺转录因子-1（ TTF-1）及 p63蛋白在非小细胞肺癌（ NSCLC）中的表达情况，并探讨其与NSCLC病理类型、临床TNM分期及淋巴结转移之间的关系。方法：采用免疫组化法检测156例NSCLC患者术后病理组织中COX-2、TTF-1、p63蛋白的表达水平。结果：COX-2、TTF-1及p63蛋白在NSCLC组织中的阳性表达率分别为94.5%（137/145）、61.0%（72/118）、53.5%（54/101）。在肺腺癌和鳞癌中，COX-2阳性表达率无显著性差异（p>0.05），而TTF-1和 p63蛋白阳性表达率具有显著统计学意义（ p<0.001）。COX-2和p63蛋白阳性表达率与NSCLC患者TNM 临床分期、淋巴结转移均无统计学差异（ p>0.05）。在Ⅲ-Ⅳ期或有淋巴结转移的NSCLC病人中，TTF-1阳性表达率显著高于Ⅰ-Ⅱ期或无淋巴结转移的病人（ p<0.01）。结论：在NSCLC病人中，TTF-1和p63蛋白的表达可能分别是腺癌和鳞癌的有效生物标志物，TTF-1的阳性表达可能与肺癌的浸润、侵袭和转移相关。