microRNA -200c 预测肺腺癌患者对吉非替尼的敏感性及其机制

摘要

目的：探讨 microRNA -200c 的表达对肺腺癌患者吉非替尼敏感性的影响及其机制。方法：收集38例 EGFR 基因敏感突变且选用吉非替尼初治的肺腺癌患者外周血，采用 real - time PCR 法检测 microRNA -200c 的表达量，MSP 法检测 microRNA -200c 启动区的甲基化状态。结果：对吉非替尼敏感的29例患者均存在 microRNA -200c 的相对高表达，其启动子区为非甲基化状态；耐药的9例患者存在 microRNA -200c 相对低表达，启动子区为甲基化状态。结论：肺腺癌患者 microRNA -200c 的表达与吉非替尼敏感性相关，作用机制可能为其启动子区的甲基化状态。%Objective:To explore the effect of microRNA - 200c expression on receiving gefitinib in patients with lung adenocarcinoma and its mechanisms. Methods:Peripheral blood in 38 patients with EGFR gene sensitive muta-tion and initial treatment options of gefitinib was used. The expression levels of microRNA - 200c were evaluated by real - time PCR. The methylation of microRNA - 200c promoter region was evaluated by methylation - specific PCR (MSP). Results:Twenty nine patients who were sensitive to gefitinib highly expressed microRNA - 200c with promot-er unmethylated,9 patients who were insensitive to gefitinib expressed low levels of microRNA - 200c with promoter methylated. Conclusion:microRNA - 200c expression in patients with lung adenocarcinoma has relation with the sen-sitivity to gefitinib. Its mechanisms may relate to methylation status of microRNA - 200c promoter region.