目的：研究肝癌靶向 SEA/ CD80共表达腺病毒（A - S - C）在小鼠体内的分布。方法：局部注射 A - S%Objective:To investigate the preclinical distribution of hepatoma - targeting recombinant co - expres-sion adenovirus vector of SEA and CD80 in mice. Methods:The mice were local injected with A - S - C. The serum and excrement concentration of adenoviruses in BALB/ c mice was tested by RT - PCR in different time point. The tissue distribution of adenoviruses in BALB/ c mice was detected by immunohistochemistry. The expression of SEA and CD80 was detected in tumor tissue of NOD/ SCID mice by Western blot. Results:After BALB/ c mice were injected with A - S - C,the serum and excrement concentration of adenoviruses in BALB/ c mice was rising rapidly,the peak appeared in the third and fifth day apart respectively,and then quickly reduced,disappeared in the 14 day. Immuno-histochemical results showed that adenoviruses were mainly distributed in the liver tissue in mice,other tissue were all the negative results. Western blot results showed that the expression of SEA was up to the peak in the third day,and gradually reduce,heaven to a minimum level to 14 day. Conclusion:The pharmacokinetic methods of A - S - C in mice was established. The results showed that A - S - C metabolic stability in mice,the tumor tissue could efficiently express SEA and CD80. Meanwhile,preliminary results show there is no obvious side effect to normal organs.
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