激活转录因子2表达与非小细胞肺癌患者预后不良的关系

摘要

目的：检测激活转录因子2（ATF2）/磷酸化激活转录因子2（p - ATF2）在非小细胞肺癌（NSCLC）中的表达，探讨其与 NSCLC 患者预后的关系。方法：采用逆转录 RCR（RT - PCR）及蛋白质免疫印迹（Western blot）方法检测 ATF2在 NSCLC 细胞系和新鲜肿瘤组织中的表达情况。免疫组织化学方法检测石蜡包埋的NSCLC 及临近非肿瘤组织中 ATF2和 p - ATF2的表达情况。结果：与对照细胞相比，NSCLC 细胞系中 ATF2表达显著上调（P <0.01）。与临近非肿瘤组织相比，NSCLC 组织中 ATF2表达显著上调（P <0.01）。ATF2表达与患者 TNM 分期（P =0.002）、肿瘤大小（P =0.018）及转移（P =0.027）密切相关。69.8%（60/86）的NSCLC 患者 p - ATF2表达显著上调。Kaplan - Meier 生存分析显示 ATF2和 p - ATF2表达上调 NSCLC 患者的总生存率显著低于低表达患者（P <0.01，P <0.05）。结论：NSCLC 组织中 ATF2和 p - ATF2表达均上调，其过表达与 NSCLC 患者预后不良密切相关。%Objective:To analyze the association between ATF2 / phosphorylated(p)- ATF2 expression and prog-nosis of NSCLC patients. Methods:Western blot and reverse transcription - quantitative polymerase chain reaction (RT - PCR)were used to detect the expression of ATF2 in fresh NSCLC tissue samples and NSCLC cell lines. Immu-nohistochemistry(IHC)was used to identify the expression and location of ATF2 and p - ATF2(threonine 71)in paraffin - embedded sections of NSCLC and adjacent normal tissue. Results:ATF2 was significantly overexpressed in the NSCLC cells and fresh NSCLC tissues compared with the control cells and samples(both P < 0. 01). ATF2 ex-pression levels were significantly increased in tumor tissues compared to normal tissues,ATF2 was located in both cy-toplasm and nucleus. ATF2 expression was closely associated with adverse clinical characteristics such as TNM stage (P = 0. 002),tumor size(P = 0. 018)and metastasis(P = 0. 027). Nuclear p - ATF2 staining was positive in 60 / 86 samples of NSCLC. Kaplan - Meier survive analysis indicated that patients with high levels of ATF2 and p - ATF2 ex-pression had a significantly shorter overall survival than that of patients with low ATF2 and p - ATF2 expression(P <0. 01 and P < 0. 05,respectively). Conclusion:Both ATF2 and p - ATF2 were significantly overexpressed in NSCLC tissues,and ATF2 and p - ATF2 overexpression predicted significantly worse outcomes for patients with NSCLC.