Survivin、CTNNB1和 hTERT mRNA 水平与胃癌药物敏感度的关系

摘要

Objective:To investigate the sensitivity of gastric cancer to docetaxel,vinorebine,irinotecan and oxali-platin,and then to assess the relationship between Survivin,CTNNB1,hTERT mRNA expression and the chemosensi-tivity.Methods:Histoculture drug response assay was used to assess the chemosensitivity of 20 gastric cancer samples to docetaxel,vinorebine,irinotecan and oxaliplatin.Real -time reverse transcription polymerase chain reaction was used to determine the expression levels of Survivin,hTERT and CTNNB1.Results:The best test concentrations of vi-norebine,irinotecan and oxaliplatin for HDRA were 20,80 and 40μg/ml,repectively.The accuracy for CTNNB1 to predict the sensitivity to docetaxel and vinorebine was 70% and the accuracy for Survivin to predict the sensitivity to oxaliplatin was 70%.Conclusion:CTNNB1 may afford to predict the chemosensitivity to docetaxel and vinorebine, while Survivin may predict the chemosensitivity to oxaliplatin in gastric cancer.%目的：初步探讨 Survivin、CTNNB1、hTERT 表达与胃癌药物敏感度的关系。方法：采用三维立体组织培养药物反应性试验检测20例胃癌组织多西紫杉醇、异长春花碱、伊立替康和奥沙利铂四种药物的敏感度， RT －PCR 方法检测胃癌组织 Survivin、CTNNB1和 hTERT mRNA 表达水平。结果：异长春花碱、伊立替康和奥沙利铂三种药物最佳检测浓度分别为20μg／ml、80μg／ml 和40μg／ml。CTNNB1 mRNA 表达用于判断多西紫杉醇和异长春花碱敏感度的准确率为70％、Survivin mRNA 表达用于判断奥沙利铂敏感度的准确率为70％。结论：CTNNB1表达水平和多西紫杉醇与异长春花碱敏感度相关，Survivin 表达水平与奥沙利铂敏感度有关。