SISH在乳腺癌HER-2基因检测中的应用价值

摘要

目的：探讨全自动银染原位杂交（SISH）方法在乳腺浸润性导管癌组织 HER -2基因检测中的应用价值。方法：分别采用免疫组化（IHC）、荧光原位杂交（FISH）、银染原位杂交（SISH）方法对105例乳腺浸润性导管癌组织中的 HER -2基因进行检测。结果：HER -2的 IHC 检测（-）、（1+）、（2+）、（3+）者与 FISH检测扩增的符合率分为100%、12.5%、46.5%、89.5%；SISH 和 FISH 检测结果的符合率为100%。结论：SISH是一种快速、准确且重复性高的 HER -2基因检测方法，可作为 FISH 的替代技术。%Objective:To evaluate HER - 2 gene expression of invasive breast carcinoma tissues determined by silver enhanced in situ hybridization(SISH)technique. Methods:Immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)and SISH was used for HER - 2 gene detection of 105 cases. Results:The coincidence rates of IHC( - ),(1 + ),(2 + ),(3 + )with FISH detection about HER - 2 gene were 100% ,12. 5% ,46. 9% , 89. 5% ,respectively. The coincidence rate of SISH with FISH about HER - 2 gene amplification were 100% . Conclu-sion:SISH is a rapid,accurate and reproducible detection methods for HER - 2 gene. It is a potenial alternative meth-od of FISH.