目的：建立可用于活体成像的小鼠大肠癌肝转移移植瘤模型。方法：利用慢病毒将荧光素酶表达载体转染至人肠癌 LoVo 细胞株，经嘌呤嘧啶筛选获得稳定表达荧光素酶的细胞克隆。进行裸鼠脾脏注射，采用脾脏注射切脾法和保脾法两种方法制造结肠癌肝转移模型，比较小鼠肝转移情况，并对肝脏组织进行 HE染色，分析肿瘤细胞的生长分布特点。结果：建立了肠癌细胞株的荧光素酶基因稳定表达的亚克隆，两组经脾注射方法肝转移率均为65%以上，具有可操作性，切脾组具备更多的肝脏转移数及更高的肝脏成瘤率。结论：成功构建了可用于活体成像的小鼠肠癌肝转移移植瘤模型，确定了脾脏注射切脾法制造小鼠结肠癌肝转移模型是较好的造模方法。%Objective:To establishment liver metastases of colorectal cancer xenograft mouse model in vivo ima-ging. Methods:Using a lentivirus vector expressing luciferase transfected into LoVo colon cancer cell lines,the purine pyrimidine screened stably expressing luciferase cell clones. To establish manufacturing cancer metastasis model. Re-sults:The establishment of a luciferase gene colon cancer cell lines stably expressing subclones,two injection methods spleen metastasis rates were 65% . Splenectomy group with more number of liver metastases and a higher rate of liver tumor. Conclusion:This study successfully constructed mouse xenograft model of colorectal cancer liver metastasis in vivo imaging it can be used to determine the method of manufacturing the spleen injection splenectomy mouse colon cancer liver metastasis model is a better modeling methods.
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