RASSF4的异常表达在非小细胞肺癌中的意义

摘要

Objective:To explored the expression pattern and significance of RASSF4 in NSCLC specimens. Methods:Samples were tested by immunohistochemistry and Western Blot.Results:RASSF4 was downregulated in 41.57%(37 /89)NSCLC tissues,and the abnormal expression of RASSF4 was significantly associated with advanced TNMstage(P =0.005),positive nodal status(P =0.024),and poor prognosis(P =0.018).We examined RASSF4 protein expression in normal lung epithelial cell line and lung cancer lines,and found that RASSF4 expression was downregulated in four of seven(57.14%,4 /7)lung cancer cell lines compared with normal bronchial epithelial cells. Conclusion:Abnormal Pygo2 protein expression may be a marker for advanced NSCLC.%目的：探讨 RASSF4在非小细胞肺癌中的表达模式和意义。方法：应用免疫组化及 Western Blot 方法，检测并分析89例非小细胞肺癌样本中 RASSF4的表达情况及其与临床病理因素的相关性。结果：在非小细胞肺癌组织中，41．57％（37／89）出现 RASSF4的表达下调，并且其异常表达与患者的高 TNM 分期（P＝0．005）、淋巴结转移（P ＝0．024）及患者不良预后（P ＝0．018）相关。在肺癌细胞系中，我们在部分细胞系（57．14％，4／7）中发现了 RASSF4的异常表达。结论：RASSF4蛋白的弱表达可能是判断非小细胞肺癌恶性程度的重要标志之一。