乳腺导管内增生性病变中 ERα基因启动子甲基化的探讨

摘要

Objective:To discuss the promoter methylation of estrogen receptor -alpha in breast intraductal prolif-erative lesions which related to the risk of breast invasive ductal cancer.Methods:The methylation of ERαpromoter was investigated in genomic DNA of 140 breast intraductal proliferative lesions(40 cases of usual ductal hyperplasia, 63 cases of atypical ductal hyperplasia,37 cases of ductal carcinoma in situ).Results:ERαmethylation was observed in 43.2%(16 /37)ductal carcinoma in situ,which was significantly lower than in usual ductal hyperplasia(55.0%, 22 /40)and was significant higher than in 11.1%(7 /63)atypical ductal hyperplasia(P <0.05).Conclusion:The methylation of ERαin breast carcinogenesis was characterized by constant change.Perhaps it was not the initiating factor in breast carcinogenesis of different molecular subtype,but it affected the biological behavior of cells.%目的：研究与乳腺浸润性导管癌风险密切相关的导管内增生性病变中 ERα基因启动子区甲基化情况。方法：用甲基化特异性 PCR 研究140例乳腺导管内增生性病变（UDH 40例、ADH 63例、DCIS 37例）的ERα第1、3、4、5启动子甲基化情况。结果：55．0％（22／40）UDH 出现 ERα基因启动子甲基化，11．1％（7／63） ADH 出现 ERα基因启动子甲基化，43．2％（16／37）DCIS 出现 ERα基因启动子甲基化，三者间差异显著（P ＜0．05）。结论：ERα基因启动子甲基化在乳腺癌的发生发展中是动态变化的，也许其并非是所有分子亚型乳腺癌的始动因素，但这个过程对于细胞的生物学行为改变是非常重要的。