Nav1．7与L－型钙离子通道在宫颈癌中的表达及临床意义

摘要

目的：探讨钙离子通道1．7亚型（Nav 1．7）与 L －型钙离子通道在子宫颈癌中的表达及临床意义。方法：采用逆转录－聚合酶链反应（RT －PCR）方法检测60例宫颈癌组织、10例经放化疗前后的宫颈癌组织、10例正常宫颈组织中的 Nav 1．7与 L －型钙离子通道的表达。结果：Nav 1．7在宫颈癌中平均值为1．6403，在正常宫颈组织中不表达，两者相比有显著性差异（P ＜0．01）。Nav 1．7 mRNA 在高分化癌、中分化癌及低分化癌中的表达分别为0．9601、0．9918及1．9014，低分化组 Nav 1．7 mRNA 表达高于高、中分化组，差异有统计学意义（P ＜0．05）。Nav 1．7 mRNA 在宫颈癌Ⅰ－Ⅱ期的表达为0．9867，在宫颈癌Ⅲ－Ⅳ期中的表达为1．8993，差异有统计学意义（P ＜0．05），Nav 1．7 mRNA 在放化疗前的宫颈癌组织中表达为1．4010与放化疗后的0．3001相比，有显著性差异（P ＜0．01）。L －型钙离子通道α1 C 亚单位 mRNA（L －Ca2＋α1 C mRNA）在正常宫颈组织中与宫颈癌组织中的灰度值的平均值为0．0914及0．3573，差异有统计学意义（P ＜0．05）。L －Ca2＋α1 C mRNA 在高分化癌、中分化癌及低分化癌中的表达分别为0．2912、0．3043及0．4935，差异有统计学意义（P ＜0．05）。L －Ca2＋α1 C mRNA 在宫颈癌Ⅰ－Ⅱ期中的表达为0．4754，在宫颈癌Ⅲ－Ⅳ期中的表达为0．8337，差异有统计学意义（P ＜0．01）。L －Ca2＋α1 C mRNA 在放化疗前的宫颈癌组织中的表达为0．5316与放化疗后的0．1024相比，有显著性差异（P ＜0．01）。结论：Nav 1．7与 L －型钙离子通道在宫颈癌中高表达，与宫颈癌的不良预后有关。放化疗可降低 Nav 1．7与 L －型钙离子通道基因的表达，与疗效相关。%Objective:To explore expression and clinical significance of Nav 1.7 and L -type calcium channel subunit α1 C in cervical cancer.Methods:Using reverse transcription -polymerase chain reaction (RT -PCR)meth-ods to detect expression of Nav 1.7 and L -type calcium channel subunit α1 C in 60 cases of cervical cancer tissue,10 cases of cervical cancer who before and after the radiation and chemotherapy group and 10 cases of normal cervical tissue.Results:The ratio of gray level of Nav 1.7 mRNA was 1.640 3 in cervical cancer,but no expression in normal cervix,in which there was significantly difference(P <0.05).The midterm expression of Nav 1.7 mRNA in high dif-ferentiation of carcinoma,differentiation of carcinoma and poorly differentiated carcinoma were 0.960 1,0.991 8 and 1.901 4,respectively,there was significantly difference(P <0.05).The midterm expression of Nav 1.7 mRNA in cervical cancer of stageⅠ -Ⅱwas 0.986 7,while in cervical cancer of stage Ⅲ -Ⅳ was 1.899 3,and the difference was statistically significant (P <0.01).Nav 1.7 was higher before radiotherapy and chemotherapy (1.4010)than that after radiochemotherapy (0.300 1)and the difference was statistically significant(P <0.01).L -type calcium chan-nel subunit α1 C mRNA in normal cervical tissues and cervical cancer tissue of the grey value of ratio were 0.091 4 and 0.357 3,respectively,there was significantly difference(P <0.05).The midterm expression of L -type calciu mchannel subunit α1 C mRNA in high differentiation of carcinoma,differentiation of carcinoma and poorly differentia-ted carcinoma were 0.291 2,0.304 5 and 0.493 5 respectively,there was significantly difference(P <0.05).The midterm expression of L -type calcium channel subunit α1C mRNA in cervical cancer of stageⅠ -Ⅱ was 0.475 4, while in cervical cancer of stage Ⅲ -Ⅳ was 0.833 7,and the difference was statistically significant (P <0.01).L -type calcium channel subunit α1 C mRNA was higher before radiotherapy and chemotherapy (0.531 6)than that after radiochemotherapy(0.102 4)and the difference was statistically significant (P <0.01).Conclusion:L -type calci-um channel subunit α1 C and Nav 1.7 gene are involved in malignant cervical cancer cell proliferation.L -type calciu mchannel subunit α1 C and Nav 1.7 gene are associated with the malignant progression,clinical staging and pathologic stage of cervical cancer.Cervical cancer radiation and chemotherapy can decrease the L -type calcium channel sub-unit α1 C and Nav 1.7 gene expression.