过表达 CHD5基因对肝癌细胞 SMMC －7721生物学行为的影响

摘要

Objective:To explore the effect of CHD5 on cells biological behavior in hepatocellular carcinoma cell line SMMC -7721.Methods:SMMC -7721 cells were transfected with pcDNA3.1(-)/CHD5 or negative control, Western blotting was performed to measure the expression of CHD5 protein,cell proliferation,apoptosis,migration and invasion was determined by CCK -8 assay,flow cytometry and transwell assay,respectively.Results:CHD5 was up-regulated after transfected with pcDNA3.1(-)/CHD5 in SMMC -7721 cells compared with NC(P <0.01),inhibi-ted cells proliferation(P <0.05),increased cells apoptosis(P <0.05),and the ability of invasion and migration was significantly increased(P <0.05).Conclusion:Overexpression of CHD5 in SMMC -7721 cells may suppress cells proliferation,invasion and migration,induce cells apoptosis.%目的：探讨过表达 CHD5基因对人肝癌细胞 SMMC －7721生物学行为的影响。方法：采用脂质体法将 CHD5过表达质粒及对照质粒分别转染 SMMC －7721细胞，Western blotting 检测 SMMC －7721细胞中CHD5蛋白的表达情况，CCK －8法检测 SMMC －7721细胞生长增殖能力的变化，流式细胞仪检测细胞凋亡，Transwell 实验检测细胞迁移和侵袭能力的变化。结果：转染 CHD5过表达质粒后，SMMC －7721细胞 CHD5蛋白表达水平显著增高（P ＜0．01），抑制了细胞增殖、迁移和侵袭（P ＜0．05），促进了细胞凋亡（P ＜0．05）。结论：过表达 CHD5基因可抑制 SMMC －7721细胞的增殖、迁移和侵袭并诱导其凋亡。