首页> 中文期刊> 《现代肿瘤医学》 >TOP2A 在非小细胞肺癌中的高表达促进肿瘤细胞的增殖和侵袭能力

TOP2A 在非小细胞肺癌中的高表达促进肿瘤细胞的增殖和侵袭能力

         

摘要

目的:探讨编码拓扑异构酶Ⅱα的基因 TOP2A 在非小细胞肺癌组织中的表达情况及其生物学功能。方法:采用荧光定量 PCR 方法检测102例非小细胞肺癌及其癌旁组织中 TOP2A 的 mRNA 表达水平,Western blot 检测其中5对组织中 TOP2A 蛋白表达水平,分析 TOP2A 表达与非小细胞肺癌主要临床病理特征的相关性。干扰非小细胞肺癌细胞株 A549和 HCC827中 TOP2A 的表达,MTT 法检测其表达对肿瘤细胞增殖的影响,Transwell 法检测 TOP2A 表达下调对肿瘤细胞侵袭能力的影响。应用流式细胞术检测 TOP2A 敲降后细胞的凋亡率和细胞周期。结果:TOP2A 在非小细胞肺癌组织中的表达水平相较癌旁组织明显上升(P <0.05),干扰 TOP2A 的表达会抑制非小细胞肺癌细胞的增殖和侵袭。流式细胞凋亡率实验结果显示转染 siTOP2A 后的非小细胞肺癌细胞早期凋亡率增加;流式周期分析显示 siTOP2A 转染后的细胞 S 期发生阻滞抑制细胞增殖。同时,TOP2A 表达水平与非小细胞肺癌的肿瘤分期、淋巴结转移密切相关(P <0.05)。结论:TOP2A 在非小细胞肺癌中表达上调,与肿瘤增殖和侵袭等生物学行为密切相关。%Objective:To investigate the expression and effects of topoismerase Ⅱ alpha(TOP2A)in non -small cell lung cancer(NSCLC)and the biological function.Methods:The expression level of TOP2A was detected in 102 cases of NSCLC tumor tissues and their corresponding adjacent normal tissues by Real time fluorescent quantitative PCR(qRT -PCR).Western blot assay was used to detect the protein level of TOP2A in five pairs of the tissues a-bove.The relationship between the expression of TOP2A and the NSCLC clinicopathological features was analyzed as well.Interference sequence of TOP2A was designed and transfected into A549 and HCC827 cells.Cell proliferation of A549 and HCC827 was analyzed by MTT.Transwell assay was employed to examine the invasiveness of the transfected cells.The cell apoptotie rate and cell cycle was assayed by flow cytometry.Results:Both mRNA and protein level of TOP2 A in NSCLC tissues was significantly higher than that in their corresponding adjacent normal tissues (P <0.05).Interference of TOP2A inhibited the proliferation and invasion of A5 49 and HCC827 cells.The flow cytom-etry assay revealed that most transfected cells were blocked in S phase ,while cell apoptosis increase(P <0.05). Overexpression of TOP2A in NSCLC tissues was associated with TNMstage and lymph node metastasis(P <0.05).Conclusion:TOP2A was upregulated in NSCLC tissues and promote cell proliferation and invasion.

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