CTLA-4基因多态性-318T/C,CT60G/A,+49G/A与结直肠癌易感性的相关分析

摘要

Objective:To explore the relationship between cytotoxic T-lymphocyte antigen-4 (CTLA-4)gene polymorphisms-318 T/ C,CT60 G/ A,+ 49 G/ A and susceptibility to colorectal cancer,and provide theoretical basis for the early forecasting of colorectal cancer.Methods:The data of 167 cases of colorectal cancer patients were select-ed as experimetal group,and 245 cases of healthy people were also recruited as control group.Peripheral blood of each participant was collected and genomic DNA was extracted,and then,CTLA-4-318 T/ C,CT60 G/ A,+ 49 G/ A gene polymorphisms were detected by PCR and direct sequencing.The frequences of-318 T/ C,CT60 G/ A,+ 49 G/A genotypes and alleles were compared and the relationship between CTLA-4-318 T/ C,CT60 G/ A,+ 49 G/ A gene polymorphisms and colorectal cancer were analysed.Results:Compared with control group,inregular diet,defeca-tion habits and basic diseases(polyp,inflammation)might be the high risk factors of colorectal cancers.Compared with patients carrying + 49 AA genotype,the risk of patients carrying + 49 GG genotype was OR = 1.698.Mean-while,the frequencies of CTLA-4 + 49 G allele carriers were significantly higher in patients than in controls (OR =0.669,P = 0.005).The distribution of CTLA-4 + 49 G/ A genotpes in different stage of colorectal cancer patients had strong statistical differences(P< 0.05).Conclusion:CTLA-4 + 49 G/ A genotype had strong correlation with the occurrence of colorectal cancer.%目的:探讨细胞毒性T淋巴细胞相关抗原-4(cytotoxic T lymphocyte antigen 4,CTLA-4)基因多态性-318 T/C,CT60 G/A,+49 G/A与结直肠癌发生的相关性,为早期预测结直肠癌的发生提供理论依据.方法:自2016年7月至2017年3月,共有167例结直肠癌患者纳入该研究,并选取同期体检健康者245例作为对照.抽取空腹外周静脉血提取DNA后,采用聚合酶链式反应(PCR)技术扩增后直接测序法检测CTLA-4-318 T/C,CT60 G/A,+49 G/A基因位点的多态性,比较两组-318 T/C,CT60 G/A,+49 G/A基因型发生的频率、不同等位基因的分布,分析CTLA-4-318 T/C,CT60 G/A,+49 G/A基因多态性与结直肠癌易感性的关系.结果:与对照组相比,不规律的饮食、排便及肠道基础疾病(息肉、炎症)均可能是结直肠癌患者的高危因素.与携带CTLA-4+49 AA基因型患者相比,携带CTLA-4+49 GG基因型患者发生结直肠癌的危险性为OR=1.698;同时,疾病组+49 G等位基因频率显著高于对照组(OR=0.669,P=0.005).不同CTLA-4+49 G/A基因型在不同分期结直肠癌患者中的分布具有明显统计学差异(P<0.05).结论:CTLA-4+49 G/A基因多态性与结直肠癌的发生有关.