宫颈癌ICAM-1的表达与微淋巴管密度的关系及其对预后的评估价值

摘要

Objective:To investigate the correlation of intercellular adhesion molecule-1 (ICAM-1) expression and microlymphatic vessel density (MLVD) and its prognostic value.Methods:The expressions of ICAM-1 and D2 -40 in 94 specimens of intratumoral and peritumoral cervical cancer and 20 specimens of normal cervix were detec ted by immunohistochemistry and MLVD were quantified.Their correlations to survival time were analyzed.Double labeling immunohistochemistry was used to show D2-40 immunoreactivity to be specific for lymphatic vessels.Resuits:The positive rate of ICAM-1 in cervical cancer tissues was 63.83％ (60/94).The MLVD of intratumoral,peritumoral and normal cervical were (2.89 ± 0.57),(8.59 ± 1.38) and (7.98 ± 1.48),respectively.Non-parametric analysis showed significant correlation between positive expression of ICAM-1 and clinical-pathological parame ter such as International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis (P ＜ 0.05).Higher intratumoural MLVD correlated significantly with late FIGO stage and presence of lymph node metastasis (P ＜ 0.05).MLVD of cervical cancer patients with ICAM-1 positive expression was significantly higher than that of negative expression (P ＜ 0.05).Conclusion:There is a correlation between ICAM-1 and lymphangiogenesis,which maybe participate in the invasion and metastasis of cervical cancer.ICAM-1 can be used as a valuable marker to evaluate the prognosis in patients with cervical cancer,and it is more significant to combine ICAM-1 with MLVD.%目的:探讨宫颈癌细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)的表达与微淋巴管密度(microlymphatic vessel density,MLVD)的相关性及二者对宫颈癌预后的评估价值.方法:采用免疫组化法检测ICAM-1、D2-40在94例宫颈癌组织、癌旁组织及20例正常宫颈组织中的表达并计数MLVD,分析上述指标与预后的关系,并采用免疫组化双重染色鉴别微淋巴管和微血管.结果:宫颈癌组织ICAM-1阳性率为63.83％ (60/94).肿瘤中心处、宫颈癌旁、正常宫颈组织中MLVD分别为(2.89±0.57)、(8.59±1.38)及(7.98±1.48).非参数检验显示ICAM-1阳性表达与宫颈癌国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)分期及淋巴结转移有关(P＜0.05);癌组织MLVD与FIGO分期及淋巴结转移密切相关(P＜0.05);ICAM-1阳性表达者MLVD明显高于阴性表达者(P＜0.05).结论:ICAM-1与宫颈癌淋巴管的生成相关,其可能参与了宫颈癌的侵袭与转移;ICAM-1对宫颈癌预后有评估价值,ICAM-1联合MLVD的预后评估意义更大.