长链非编码RNA LINC01018在非小细胞肺癌中的表达与意义

摘要

Objective:To investigate the expression of lncRNA LINC01018 in NSCLC to evaluate its clinical significance,and study its influence in NSCLC.Methods:Total of 60 specimens of NSCLC tissues and normal lung tissues after surgery were collected.qRT-PCR was performed for LINC01018 expression in cancerous and normal lung tissues.The correlations between the expression of LINC01018 and pathological characteristics were analyzed.pcDNA-LINC01018 was transfected to up-regulate LINC01018 expression in NSCLC cells,and the transfection efficiency was evaluated by qRT-PCR.CCK8 assay was performed to evaluate the effect of ectopic LINC01018 expression on proliferation of NSCLC cells.The expression of RAC1 protein was determined by Western blot.Results:The expression level of LINC01018 was down-regulated in tumor tissue compared with the paired normal lung tissue(P<0.05).The expression of LINC01018 was associated with patients gender,tumor size,degree of differentiation,lymphatic metastasis and the distal metastasis (P<0.05).Meanwhile,over expression of LINC01018 reduced the expression of RAC1 and the proliferation of NSCLC cells were decreased.Conclusion:The expression of LINC01018 is obviously decreased in NSCLC patients with female patients,large tumor size,high clinical stage and advanced histological grade.LINC01018 may inhibit cell proliferation by affecting the expression of RAC1 proteins.%目的:探讨长链非编码RNA LINC01018在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达水平和临床意义,以及LINC01018对NSCLC细胞增殖的影响.方法:用qRT-PCR检测60例NSCLC组织和癌旁肺组织中LINC01018的表达,分析LINC01018表达与非小细胞肺癌临床特征的相关性.通过转染pcDNA-LINC01018上调 LINC01018的表达水平,采用qRT-PCR方法检测重组质粒在细胞中的表达水平.CCK8检测NSCLC细胞增殖能力的变化,Western blot法检测LINC01018对RAC1蛋白的影响.结果:相比癌旁肺组织,在NSCLC组织中LINC01018的表达出现显著下调.肺癌组织中LINC01018的表达水平与患者性别、肿瘤大小、分化程度及淋巴转移和远端转移明显相关(P<0.05).LINC01018的过表达能降低肺癌细胞的增殖能力并下调RAC1的表达.结论:LINC01018表达随着女性患者、肿瘤直径大、分期晚及分化差而显著下调.LINC01018可通过影响RAC1,抑制NSCLC细胞的增殖.