RhoB在肾透明细胞癌中的表达及临床意义

摘要

目的:探讨RhoB在肾透明细胞癌中的表达及临床意义.方法:采用实时定量PCR、Western blot和免疫组化方法检测RhoB在肾透明细胞癌组织中的表达情况.选取60例肾透明细胞癌组织标本,详细收集病人临床病理资料,通过免疫组化方法检测RhoB在肾透明细胞癌组织中的蛋白表达水平,并分析RhoB的蛋白水平与临床病理资料的关系.结果:与对应瘤旁肾组织相比,RhoB的mRNA及蛋白表达水平在肾透明细胞癌组织标本中明显降低;RhoB的蛋白表达水平在不同年龄、性别组间差异无统计学意义(P>0.05),而在肿瘤直径大小、T分期、临床分期和组织分级间差异有显著统计学差异(P<0.05).结论:RhoB的表达降低可能在肾透明细胞癌的肿瘤发生中发挥作用,且其表达下降可能与肾透明细胞癌的恶性进展有关.%Objective:To investigate the RhoB expression and significance in clear cell renal cell carcinoma(ccRCC).Methods:The expression of RhoB was detected in ccRCC specimens and their corresponding adjacent renal tissue samples using real-time PCR,Western blot and immunohistochemistry respectively.And the expression of RhoB protein level was detected by immunohistochemistry in 60 cases of ccRCC tissues.The relationship of RhoB protein expression with the tumor pathologic parameter was analyzed.Results:The expression of RhoB in mRNA and protein levels was low-expressed in ccRCC surgical specimens compared with adjacent renal tissues.The RhoB protein expression level was significantly associated with the tumor size,pT stage,TNM stage and Fuhrman tumor grade(P<0.05),but not age and gender(P>0.05).Conclusion:The low-expressed of RhoB may play an important role in the carcinogenesis and development of ccRCC.